Abstract:
Artificial insemination (AI) is the top of the list breeding technique to deposit superior genetic male germplasm in Beetal does. This is the first study in Pakistan in Beetal does, in which the efficacies of modern laparoscopic vs. conventional cervical AI techniques are investigated in estrus synchronized Beetal does during the peak breeding season. In experiment I, forty-eight nonpregnant Beetal does in 2nd lactation were estrus synchronized with 7-day progesterone- based protocol. Sponge containing 60 mg medroxyprogesterone acetate (MAP: ESPONJAVET® Hirpa Laboratories Spain) inserted intravaginally for 7 days, 75 µg of cloprostenol sodium (1mL Dalmazine FATRO® Italy) and 300 IU of Pregnant Mare Serum Gonadotrophin (PMSG: 3mL OVISER Hirpa Laboratories Spain) were administered intramuscularly on day 7. At 16 hours after standing estrus (standing estrus detected with aproned teaser buck), laparoscopic AI (LAI) was performed in twenty-one and cervical AI in nineteen does by using fresh semen of Beetal buck. In experiment II, fortynine non- pregnant Beetal does in 2nd lactation were estrus synchronized with intravaginal sponge containing 60 mg MAP for 11 days. On day 9, 75 µg of cloprostenol sodium and 300 IU of PMSG was administered intramuscularly. At 56 hours after sponge removal, twenty does were inseminated through LAI and twenty-three through cervical AI with frozen thawed Beetal buck spermatozoa. Pregnancy was diagnosed at 50 days post AI through trans-abdominal ultrasonography. The data on pregnancy rate per AI and prolificacy were analyzed through Chi- square test of association and t test respectively by using Minitab software. The probability level having P value less than 0.05 was considered significant. No significant difference was recorded between 7-day and l1-day estrus synchronization protocols with respect to estrus response (83.33 vs 87.75%, respectively) and estrus interval (43.8 ± 1.99 vs 40.17 ± 1.43 hours, respectively). In both experiments, the pregnancy rates per AI was significantly higher in LAI group as compared to cervical AI by using fresh (71.42 vs 26.1 %, respectively) and frozen thawed Beetal buck spermatozoa (60 vs 42.1 %, respectively). The prolificacy did not differ significantly between LAI and Cervical AI groups of Experiment I (1.26 vs. 1.25, respectively) and experiment II (1.25 vs. 1.33, respectively). In conclusion, the findings of this study indicate that LAI technique improves the outcome of controlled breeding in Beetal does during the peak breeding season.
Page(s):
217-217
DOI:
DOI not available
Published:
Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
Controlled Breeding Technology
,
Beetal does
,
Laparoscopic Artificial Insemination
,
Pregnancy rate per AI