Alleviation of phyto-toxic effects of chromium by inoculation of chromium (VI) reducing Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium Rb-2. | [International Journal of Agriculture and Biology • 2015]

Author(s):

1. Rida Batool: Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan; MEM-group, Department of Biosciences, University of Helsinki, Finland

2. Kim Yrjälä: Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan

3. Shahida Hasnain: Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan

Abstract:

Two indigenous Cr(VI) reducing bacterial strains, Pseudomonas aeruginosa Rb-1 and Ochrobactrum intermedium Rb-2 were used in this study. Mono as well as mixed cultures of these strains were used to inoculate the seeds of wheat under chromium (III and VI) stressed and chromium free conditions. Inoculated seeds were irrigated with industrial waste water and distilled water. Industrial waste water in combination with Cr(VI) exhibited more adverse effects on the growth as well as biochemical attributes of wheat. Seed germination was severely affected by the application of CrCl3 and K2CrO4 irrigated with industrial waste water and distilled water. Bacterial inoculation caused significant enhancement in percentage seed germination when compared with non-inoculated control. Application of chromium salts (III and VI) led to decrease in seedlings length but bacterial inoculation caused improvement in it as compared to respective non-inoculated treatment. Chromium (III and VI) stress in combination with industrial effluent caused significant increase in auxin content of wheat seedlings. Bacteriazation caused significant rise in peroxidase and soluble protein content under chromium (III and VI) stress and unstressed conditions. Maximum augmentation in studied growth and biochemical parameters were observed with the inoculation of bacterial strain O intermedium Rb-2. Reduction of chromium (VI) uptake by the inoculated seedlings was most prominent observation. Scanning electron microscopy (SEM) revealed the presence of large number of bacterial cells on the surface of inoculated seedlings.

Page(s): 21-30

DOI: DOI not available

Published: Journal: International Journal of Agriculture and Biology, Volume: 17, Issue: 1, Year: 2015

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