Validation and application of RP-HPLC method for the quantification of metoclopramide hydrochloride in oral formulations prepared for IVIVC studies. | [Pakistan Journal of Pharmaceutical Sciences • 2012]

Author(s):

1. Ahmad Khan: Department of Pharmaceutics, Faculty of Pharmacy, University of Karachi, Pakistan

2. Syed Baqir Shyum Naqvi: Department of Pharmaceutics, Faculty of Pharmacy, University of Karachi, Pakistan

3. Muhammad Harris Shoaib: Department of Pharmaceutics, Faculty of Pharmacy, University of Karachi, Pakistan

4. Rabia Ismail Yousaf: Department of Pharmaceutics, Faculty of Pharmacy, University of Karachi, Pakistan

5. Jallat Khan: Department of Chemistry, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

6. Muhammad Hanif: Department of Pharmaceutics, Faculty of Pharmacy, University of Karachi, Pakistan

7. Asadullah Madni: Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

Abstract:

The objective of this study is to develop sensitive and cost effective reverse phase high performance liquid chromatographic method for the estimation of Metoclopramide Hydrochloride in oral solid dosage formulations. A reverse chromatographic method was used with the mobile phase of Acetonitrile, 20m M Potassium dihydrogen phosphate buffer solution (pH 3 adjusted with orthophosphoric acid) in the ratio of 40:60. The column used was Waters C18 3.9×300mm µBondapak (RP). The flow rate of the mobile phase was 2ml/minute. The detector was set at the wavelength of 275nm.This method showed good sensitivity. The linearity was also found to be excellent (γ2=0.997) in the range of 5-75 µg/ml. No interfering peaks were observed at the retention time of Metoclopramide Hydrochloride when both placebo and blank samples were injected (Retention time =1.93min). The parameters such as specificity, linearity, range, accuracy, precision, system suitability, solution stability, detection and quantification limits were evaluated to validate this method. This method can effectively be used for quantitative analysis of Metoclopramide hydrochloride tablet formulations because of its specificity, accuracy and convenience of use.

Page(s): 135-140

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 25, Issue: 1, Year: 2012

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