Bacterial Biofilms and Ethylenediamine-N,N'-disuccinic acid (EDDS) as Potential Biofilm Inhibitory compound | [Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences • 2020]

Author(s):

1. Nimerta Kumari: Institute of Microbiology, University of Sindh, Jamshoro, Pakistan

2. Shabnam Rathore: Department of Agriculture Chemistry, Agriculture Research Institution,Tando Jam,Pakistan

3. Bushra Bano Patoli: Institute of Microbiology, University of Sindh, Jamshoro, Pakistan

4. Atif Ahmed Patoli: Institute of Microbiology, University of Sindh, Jamshoro, Pakistan

Abstract:

Bacterial biofilms are populations of microorganisms that stick to some surfaces and are embedded in selfsecreted extracellular polymeric matrixes. These synergistically controlled sessile cells exhibit different proteome than their planktonic counterpart and are often dificult to treat. They make a different mechanism to escape the antimicrobial activity and host defenses. Biofilms on indwelling prosthetic devices are a major problem in the medical field because they could lead to recurrent and systemic infections. Biofilms also result in bio-corrosion in industries and their remediation often causes environmental problems. The goal of this study is to find antimicrobial and biofilm inhibition activity of non-toxic and biodegradable economical iron-chelating compound EDDS against Staphylococcus aureus SA113 and Pseudomonas aeruginosa PAO1 strains. Broth microdilution assay was performed to find the inhibitory concentration of EDDS and biofilm assay performed in the micro-well plate to determine the biofilm inhibitory concentration of EDDS against SA113 and PAO1. The results show that a higher concentration of EDDS is required to suppress the growth of SA113 a gram-positive bacterium compare to gram-negative PAO1 strain. EDDS successfully inhibited the biofilm formation of both SA113 and PAO1 at very low concentration and significant biofilm inhibition (p<0.05) was observed at 0.78 mM and 1.56 mM EDDS concentration for PAO1 and SA113 respectively. Biodegradable ion-chelator EDDS is a good biofilm inhibitor compound for both S. aureus SA113 and P. aeruginosa PAO1 strains.

Page(s): 85-92

DOI: DOI not available

Published: Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 57, Issue: 1, Year: 2020

Keywords:

Staphylococcus aureus , biofilm inhibition , Pseudomonas aeruginosa , ion chelation , EDDS

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