Ameliorative effects of Withania coagulans and Metformin on Ovarian Morphology in Rats with Polycystic Ovarian Disease | [Journal of Islamabad Medical & Dental College • 2021]

Author(s):

1. Hira Ayaz: Department of Physiology, Foundation University,Islamabad,Pakistan

2. Abdul Samad: Department of Physiology, Pak International Medical College,Peshawar,Pakistan

3. Aiman Farogh Anjum: Department of Physiology, CMH Kharian Medical College,Kharian Cantonment,Pakistan

4. Nasar Abbas Shamsi: Department of Physiology, Foundation University,Islamabad,Pakistan

5. Sidra Arshad: Department of Physiology, Rawal Institute of Health Sciences,Islamabad Pak istan,

6. Mahvash Khan: Department of Physiology, Rawal Institute of Health Sciences,Islamabad,Pakistan

Abstract:

Background: Various medicinal herb plants are being used in place of metformin for treatment of polycystic ovarian disease for their less harmful effects. Withania coagulans (WC) is a herb known for its insulin sensitizing and weight reducing properties. The present study was done to determine the influence of aqueous extract of Withania coagulans (aqWC) and metformin on ovarian weight and ovarian folliculogenesis in polycystic ovarian disease -induced rats. Material and Methods: An experimental animal study was carried out at the Physiology Department of Islamic International Medical College, Rawalpindi from April 2016 to March 2017. Forty female Sprague Dawley rats were divided initially into two groups. Group A (Normal Control) and Disease induced group. Standardized laboratory diet was fed to Group A while the disease induced group was given standardized laboratory diet and letrozole solu tion orally (1mg/kg) for 21 days to induce Polycystic ovary syndrome, which was established by observing estrous cycle of rats. Disease induced group was then split into group B (PCOS control), C (Aqueous Withania coagulans treated) and D (Metformin treated) and observed after 14 days. Groups A and B underwent ovarian dissection after 21 days and groups C and D underwent dissection at the end of experiment (after 35 days). Independent sample t -test was used for the comparison between the control and disease induced group. Results: Group B showed a significant increase in ovarian weight in comparison to group A rats (P < .05). Treatment with Withania coagulans and metformin significantly decreased ovarian weight (P < .05) and increased primary, Graafian, antral follicular count and corpus luteum along with reduction in cystic follicular count in letrozole -induced polycystic ovarian disease rats. Improved folliculogenesis was also observed in the same groups (groups C & D) . Conclusions: Withania coagulans can be a substitute for improvement of ovarian follicular development in polycystic ovarian disease.

Page(s): 31-36

DOI: 10.35787/jimdc.v10i1.457

Published: Journal: Journal of Islamabad Medical & Dental College, Volume: 10, Issue: 1, Year: 2021

Keywords:

metformin , Estrous cycle , Polycystic ovarian disease , Withania coagulans

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views