Author(s):
1. Summiya Akram:
The Physio College of Rehabilitation Center Multan, Pakistan
2. Areesha Naveed:
The Physio College of Rehabilitation Center Multan, Pakistan
Abstract:
Pseudomonas aeruginosa is gram negative rod bacterium. It can be remoted in low number from huge sort of environment consisting of soil and water. It is main cause of death and illness in human with immunosuppressive. We take different sources from Nishtar hospital Multan. The sources were sink, tap, mop and wound with pus. These sources were collected carefully with proper PPEs. For the isolation of pseudomonas aeruginosa, first prepare MH agar and put it in petri plates and incubate for 24 hours. Then swab the petri plates to spread the sources all over the plate, then streaking is performed. Next step is serial dilution which is used to check the number of microorganisms found in the sample, then pour plate by putting the sample in petri plate again and count the colonies in colony counter. Then prepare the smear on slide and perform gram staining to observe the bacteria under microscope. Pseudomonas aeruginosa has been identified as most important cause of hospital acquired pneumonia, health care associated pneumonia. The MH agar showed green color colonies and the microscopic observation showed red color colonies. Several studies show pseudomonas aeruginosa as important pathogen in burn patients and also isolate frequent infection in wound. We have to stop the unfold of antibiotic resistance bacteria, it far severely critical to have strict antibiotic rule as a same time as surveillance programmes for multidrug resistance bacteria.
Page(s):
9-9
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Nosocomial infection
,
sterilization
,
UV radiation
,
Cross contamination
,
Follow PPEs
References:
References are not available for this document.
Citations
Citations are not available for this document.