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A comparative study of the effects of diluents and thawing temperature on rate of motility and liveability of spermatozoa stored 4 C.
Author(s):
1. Ala-Ud-Din: Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan
2. Nazir Ahmad: Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan
3. Osama I. Azawi: College of Veterinary Medical University, Baghdad, Iraq
Abstract:
Thirty ejaculates from three Friesian bulls kept at the Artificial Insemination Centre, Abu-Ghrab, Baghdad, Iraq, were used to study the effect of five diluents and two thawing temperatures on rate of motility, liveability and index of liveability of spermatozoa stored at 4°C. The five diluents comprised (A) lactose-eggyolk-glycerol, (B) fructose-eggyolk glycerol, (C) glycine-egg yolkglycerol (D) lactose-fructose-glycine-eggyolk-glycerol and (E) laiciphos-glycerol. The two thawing temperatures studied were (I) 35oC for 2.5 minutes and (ii) 0°C for 10 minutes. The best results in terms of rate of motility (50.08%) and index motility (39.67%), liveability in days (9.03) and index of liveability (2678) were obtained with diluent A, the difference being significant. Significantly higher rate of motility (48.40 V 41.63%) and index of liveability (3859 V 3050) were observed with temperature of 35°C than that of 0°C. The two thawing temperatures, however, differed non-significantly from each other in terms of liveability of spermatozoa at 4'C.
Page(s): 114-119
DOI: DOI not available
Published: Journal: Pakistan Veterinary Journal, Volume: 5, Issue: 3, Year: 1985
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