A novel Realgar-indigo Naturalis Formula More Effectively Induces Apoptosis in Nb4 Cells. | Pakistan Journal of Pharmaceutical Sciences 2019

Qinjian Xie GanSu Corps Hospital of CAPF, Lanzhou, Gansu, PR ChinaGansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, PR China

Lan Yu School of Pharmacy, Lanzhou University, Lanzhou, Gansu, PR China

Xin Wang School of Pharmacy, Lanzhou University, Lanzhou, Gansu, PR China

Zhengrong Wu School of Pharmacy, Lanzhou University, Lanzhou, Gansu, PR China

Dejuan Zhi School of Pharmacy, Lanzhou University, Lanzhou, Gansu, PR China

Zhiwang Guo Guangdong Corps Hospital of CAPF, Guangzhou, Guangdong, PR China

Tao Wu Guangdong Corps Hospital of CAPF, Guangzhou, Guangdong, PR China

Yin Sun Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, PR China

Longhe Zhao School of Pharmacy, Lanzhou University, Lanzhou, Gansu, PR China

XiaoYu Ding GanSu Corps Hospital of CAPF, Lanzhou, Gansu, PR China

Akbar Khan Department Biological Sciences Karakoram International University Gilgit, Pakistan

Hongyu Li Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, PR China ; School of Pharmacy, Lanzhou University, Lanzhou, Gansu, PR China

JingYang Northwest Minzu University, Lanzhou, Gansu, PR China

Realgar as a kind of arsenic agent is currently used to treat APL in China. The effectiveness and low toxicity of realgar have been verified, lower than arsenic trioxide. Although the therapeutic efficacy of realgar is blocked severely by its poor insolubility in water. In our lab, we addressed this problem by obtaining realgar bioleaching solution (RBS) from microbiological leaching technique. To develop a tradition Chinese medicinal formula (TCMF) for clinical application realgar is usually used with other herbs. However, treated realgar with RBS has not been evaluated in TCMF contain realgar. In the present study we used NB4 to investigate the effects of novel Realgar-Indigo naturalis formula (FRBS) on cell proliferation and apoptosis. We used MTT assay to measure anti proliferative activity of FRBS. We further study the effects of FRBS on cell growth and apoptosis according flow cytometry, DNA fragmentation assay and Fluorescence microscopy and Western blot. The results revealed that FRBS significantly inhibited growth in a dosedependent manner, and induced apoptosis in NB4 cells. NB4 cell inhibitory response to FRBS at 2µg ml-1 of arsenic concentration was twofold higher, dissimilar to RIF, and induced apoptosis more effectively. Further, a higher expression of caspase-3, caspase-9 and cytochrome C from increased from FRBS. RBS can substitute the traditional realgar powder in RIF in order to provide a novel and promising Realgar-Indigo naturalis formula to treat acute promyelocytic leukemia.