Age-dependent Cytokine Expression in Response to Foot-and-mouth Disease Virus in Bovine Peripheral Blood Mononuclear Cells | [Pakistan Veterinary Journal • 2023]

Author(s):

1. Seung-Won Yi: Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration,1500 Kongjwipatjwi-ro, Wanju 55365,Republic of Korea

2. Ngoc Anh Bui: Virology Department, National Institute of Veterinary Research,Hanoi,Vietnam

3. Hu Suk Lee: College of Veterinary Medicine, Chungnam National University,Daejeon 34134,Republic of Korea; International Livestock Research Institute,Hanoi,Vietnam

4. Vuong Nghia Bui: Virology Department, National Institute of Veterinary Research,Hanoi,Vietnam

5. Duy Tung Dao: Virology Department, National Institute of Veterinary Research,Hanoi,Vietnam

6. Trang Huyen Nguyen: Virology Department, National Institute of Veterinary Research,Hanoi,Vietnam

7. Han Gyu Lee: Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration,1500 Kongjwipatjwi-ro, Wanju 55365,Republic of Korea

8. Young-Hun Jung: Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration,1500 Kongjwipatjwi-ro, Wanju 55365,Republic of Korea

9. Tai-Young Hur: Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration,1500 Kongjwipatjwi-ro, Wanju 55365,Republic of Korea

10. Sang-Ik Oh: Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration,1500 Kongjwipatjwi-ro, Wanju 55365,Republic of Korea

Abstract:

The severity of foot-and-mouth disease virus (FMDV) infections differs between calves and cattle. Here, we compared immunological responses to FMDV in bovine peripheral blood mononuclear cells (PBMCs) from juvenile cattle (7-9 months) and calves (3-4 months). PBMCs were collected from cattle and inoculated with serotype O FMDV and incubated for 0, 1, 3, 6, 12, 24, 48, 72, and 96 h. At each time point, total RNA was isolated from PBMCs and the mRNA expression of six cytokines (IFN-?, TNF-a, IL-2, IL-4, IL-6, and IL-10) was evaluated. Th1 (IFN-? and IL-2) and Th2-related (IL-4 and IL-10) cytokine levels were more prominent in juvenile cattle than in calves. In particular, IL-2 and IL-10 from juvenile cattle were significantly (P<0.001) higher than those from calves at 48 and 24 hpi, respectively. Therefore, the juvenile cattle showed remarkable Th1 and Th2-related immune response than calves within 48 h after FMDV infection.

Page(s): 209-212

DOI: 10.29261/pakvetj/2023.003

Published: Journal: Pakistan Veterinary Journal, Volume: 43, Issue: 1, Year: 2023

Keywords:

Footandmouth disease Cattle age Cytokine expression Peripheral blood mononuclear cells Viral immunology

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