Pakistan Science Abstracts
Article details & metrics
No Detail Found!!
Anti-inflammatory and Anticancer Activity of Pteris Cretica Whole Plant Extracts.
Author(s):
1. Komal Kiran: Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan
2. Farooq Saleem: Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan
3. Sana Awan: Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan
4. Sarfraz Ahmad: Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan
5. Shoaib Ahmad: Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan
6. Mammar Arshad Malik: Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan
7. Bushra Akhtar: Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture Faisalabad, Pakistan
8. Moosa Raza: Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan
9. Sohaib Peerzada: Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan
10. Ali Sharif: Faculty of Pharmacy, The University of Lahore, Lahore, Pakistan
Abstract:
The present study focuses on the use of n-hexane, chloroform, ethyl acetate and aqueous extracts of Pteris cretica to confirm its in-vivo anti-inflammatory properties and in-vitro cytotoxic profile. The anti-inflammatory study was conducted by carrageenan-induced edema test at different doses of Pteris cretica extracts (250 and 500mg/kg body weight). At 250 mg/kg dose of chloroform, n-hexane, aqueous and ethyl acetate extracts expressed maximum anti-inflammatory activity at the 2nd hour with 29.65, 29.06, 27.55 and 26.04% respectively by inhibiting carrageenaninduced edema. Moreover, 500 mg/kg concentration of chloroform extract of Pteris cretica expressed efficient anti-inflammatory activity with 45.3% of inhibition in edema; whereas n-hexane, aqueous and ethyl acetate extracts inhibit the edema at 35.34, 29.06 and 27.55% respectively. MTT assay was used to determine the cytotoxic potential of Pteris cretica extracts on HeLa, and BHK-21 cell lines. Chloroform and aqueous extracts expressed the cytotoxic activity with IC50 of 31.48 and 34.26 µg/mL respectively against HeLa cell lines. However, both chloroform and aqueous extracts exhibit IC50 108.50 and 55.76 µg/mL against BHK-21 cell line respectively. Chloroform extract is less cytotoxic against BHK-21 cell line as compared to aqueous extract. Ethyl acetate extract was found to be toxic for both cell lines. Based upon the above results, isolation, identification and purification of phytochemicals from Pteris cretica extracts is highly recommended as a possible extension of the current work.
Page(s): 225-230
Published: Journal: Pakistan Veterinary Journal, Volume: 38, Issue: 3, Year: 2018
Keywords:
Antiinflammatory , Anticancer , Carrageenan induced edema , Pteris cretica , MTT assay , testing
References:
[1] AbbasA,IqbalZ,AbbasRZ,,2017a,Immunomodulatory activity of Pinus radiata extract against coccidiosis in broiler chicken,Pak Vet J,37,145-9
[2] AbbasA,IqbalZ,AbbasRZ,,2017b,In vivo anticoccidial effects of Beta vulgaris (sugar beet) in broiler chickens,Microb Path,111,139-44
[3] AhmadA,AlkarkhiA,HenaS,,2009,Extraction separation and identification of chemical ingredients of Elephantopus scaber L. using factorial design of experiment,Int J Chem,1,36-49
[4] AhmadF,KhanRA,RasheedS,,1992,Study of analgesic and antiinflammatory activity from plant extracts of Lactuca scariola and Artemisia absinthium,J Islamic Acad Sci,5,111-4
[5] AkhtarB,AshrafM,JaveedA,,2016,Analgesic, antipyretic and anti-inflammatory activities of Grewia asiatica fruit extracts in albino mice,Acta Pol Pharm,73,983-9
[6] AsongalemE,FoyetH,EkoboS,Ness) T. Anderson,,2004,Antiinflammatory lack of central analgesia and antipyretic properties of Acanthus montanus (,J Ethnopharmacol,95,63-8
[7] Batra P and Sharma,AK,,2013,Anti-cancer potential of flavonoids recent trends and future perspectives,Biotech,3,439-59
[8] ChenY,ChangF,LuM,,2008,New benzoyl glucosides and cytotoxic pterosin sesquiterpenes from Pteris ensiformis burm,Molecules,13,255-66
[9] CoussensLM,WerbZ,,2002,Inflammation and cancer,Nature,420,860-7
[10] El-WahabAEA,GhareebDA,SarhanEE,,2013,In vitro biological assessment of Berberis vulgaris and its active constituent, berberine antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects,BMC Complem Altern Med,13,218-
[11] GanTJ,,2010,Diclofenac an update on its mechanism of action and safety profile,Current Med Res Opin,26,1715-31
[12] GracelinDHS,BrittoA,KumarB,,2013,Qualitative and quantitative analysis of phytochemicals in five pteris species,Int J Pharm Pharm Sci,5,105-7
[13] GuptaM,MazumderU,KumarRS,,2005,Anti-inflammatory, analgesic and antipyretic effects of methanol extract from Bauhinia racemosa stem bark in animal models,J Ethnopharmacol,98,267-73
[14] HassanKU,KhaliqueA,PashaTN,,2017,Influence of Moringa oleifera decorticated seed meal on broiler performance and immunity,Pak Vet J,37,47-50
[15] HemamaliniK,NaikKOP,AshokP,,2010,Anti inflammatory and analgesic effect of methanolic extract of Anogeissus acuminata leaf,Int J Pharm Biomed Res,1,98-101
[16] HossainMH,JahanF,HowladerMSI,,2017,Evaluation of antiinflammatory activity and total flavonoids content of Manilkara zapota (linn,International J Pharm Phytopharmacol Res,2,35-9
[17] HossenMJ,UddinMB,AhmedSSU,,2016,Traditional medicine/plants for the treatment of reproductive disorders in Asia nations,Pak Vet J,36,127-133
[18] KaurR,KapoorK,KaurH,,2011,Plants as a source of anticancer agents,J Nat Prod Plant Resour,1,119-24
[19] KhasawnehMA,ElwyHM,HamzaAA,,2011,Antioxidant, antilipoxygenase and cytotoxic activity of Leptadenia pyrotechnica (forssk.) decne polyphenolic constituents,Molecules,16,7510-21
[20] LiaqatI,PervaizQ,BukhshSJ,,2016,Investigation of bactericidal effects of medicinal plant extracts on clinical isolates and monitoring their biofilm forming potential,Pak Vet J,36,159-164
[21] MannanMM,MaridassM,VictorB,,2008,A review on the potential uses of ferns,Ethnobotanical Leaflets,12,281-5
[22] MehmoodA,HamidI,SharifA,,2016,Evaluation of antiinflammatory, analgesic and antipyretic activites of aqueous and ethanolic extracts of seeds of Buchanania lanzan spreng in animal models,Acta Poloniae Pharmaceutica,73,1601-8
[23] MichaelY,DuboisC,AllenH,,2003,Wylie and church-davidson's: A Practice of Anaesthesis,,,1235-9
[24] PilottoA,FranceschiM,LeandroG,,2003,The risk of upper gastrointestinal bleeding in elderly users of aspirin and other nonsteroidal antiinflammatory drugs, the role of gastroprotective drugs,Aging Clin Experim Res,15,494-9
[25] RatheeP,ChaudharyH,RatheeS,,2009,Mechanism of action of flavonoids as anti-inflammatory agents: A review. Inflammation & allergy- drug targets (formerly current drug targets-inflammation & allergy),,8,229-35
[26] SaimaS,DastiAA,AbbasQ,,2010,Floristic D iversity during monsoon in ayubia national Park,Pak J Plant Sci,16,1-
[27] SaleemF,KhanMTJ,SaleemH,,2016,Phytochemical, antimicrobial and antioxidant activities of Pteris cretica L. (Pteridaceae) extracts,Acta Poloniae Pharmaceut Drug Res,73,1397-1404
[28] SharifA,AkhtarMF,AkhtarB,,2017,Genotoxic and cytotoxic potential of whole plant extracts of Kalanchoe laciniata by ames and mtt assay,Excli J,16,593-601
[29] SharifA,AshrafM,AnjumAA,,2016,Pharmaceutical wastewater being composite mixture of environmental pollutants may be associated with mutagenicity and genotoxicity,Environm Sci Poll Res,23,2813-20
[30] ShilS,ChoudhuryMD,,2009,Ethnomedicinal importance of pteridophytes used by reang tribe of tripura,North East India. Ethnobotanical Leaflets,13,634-43
[31] SonHL,ThaoTHP,,2014,In vitro antioxidant and anticancer properties of active compounds from methanolic extract of Pteris multifida poir leaves,European J Med Plants,4,292-302
[32] SosaS,BalickM,ArvigoR,,2002,Screening of the topical antiinflammatory activity of some central american plants,J Ethnopharmacol,81,211-5
[33] StockertJC,Blázquez-CastroA,CañeteM,,2012,MTT assay for cell viability intracellular localization of the formazan product is in lipid droplets,Acta Histochemica,14,785-96
[34] TahirM,RasheedMA,NiazQ,,2017,Evaluation of antibacterial effect of Gymnema sylvestre R.Br. species cultivated in Pakistan,Pak Vet J,37,245-250
[35] ,2002,,,,5-
Citations
Citations are not available for this document.
0

Citations

0

Downloads

32

Views