The Role of YAP-JNK in the Mitophagy, Migration, and Invasion of Gastric Cancer | [Journal of College of Physicians and Surgeons--Pakistan : JCPSP • 2023]

Author(s):

1. Feng Xiao: Department of Pathology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China

2. Min Guo: Department of Pathology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China

3. Ruiping Cao: Department of Pathology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China

4. Jue Zou: Department of Pathology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China

5. Lina Yi: Department of Pathology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China

6. Hongzhu Yan: Department of Pathology, Seventh People's Hospital of Shanghai University of TCM, Shanghai, China

Abstract:

Objective: To determine the function of the YAP-JNK-mitophagy signalling pathway in gastric cancer (GC). Study Design: An observational study. Place and Duration of Study: Seventh People's Hospital of Shanghai University of TCM (Traditional Chinese Medicine), between June 2019 and June 2021. Methodology: Tissues from 30 cases of gastric cancer and corresponding adjacent tissues were collected. RT-qPCR was employed to detect the expression of YAP and JNK in GC samples. MTT, Wound healing and Transwell assays were used to detect changes in GC cell proliferation, migration, and invasion under di erent stimulation. LC3 immuno uorescence and mitochondrial membrane potential detection were used to analyse the occurrence of mitochondrial autophagy. Results: The expression of YAP and JNK were signi cantly increased in GC tissues (p=0.024, 0.033). YAP knockdown inhibited GC cell proliferation, migration, and invasion. Further studies showed that YAP a ects GC cell function by targeting JNK. In addition, YAP-JNK signalling was found to regulate GC cell proliferation, migration, and invasion mainly through regulating the occurrence of mitophagy. Conclusion: These ndings revealed that YAP-JNK promotes the development of GC by targeting mitophagy.

Page(s): 149-152

DOI: 10.29271/jcpsp.2023.02.149

Published: Journal: Journal of College of Physicians and Surgeons--Pakistan : JCPSP, Volume: 33, Issue: 2, Year: 2023

Keywords:

Autophagy , Gastric cancer , YAP , JNK

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