Abstract:
The increasing risk of bacterial resistance to antibiotics and associated side effects has driven researchers to explore medicinal plants as natural alternatives to synthetic drugs. This study investigates the antimicrobial activities of four seeds (Trigonella foenum-graecum, Salvia hispanica, Ocimum basilicum, and Coriandrum sativum) commonly used in traditional medicine against five bacterial strains (E. coli, Klebsiella pneumoniae, Proteus mirabilis, Salmonella spp., and Staphylococcus aureus) and one fungal strain (Candida spp) isolated from fruit samples. The bacteria were identified based on cultural morphology, Gram staining, and Analytical Profile Index (API 20E) testing. The antimicrobial activity of the seed extracts was tested using ethanol, methanol, acetone and distilled water as solvents in a disc diffusion method. Ethanol extracts generally exhibited higher activity than methanol, acetone, and distilled water, except for Trigonella foenum-graecum, which showed better activity in methanol. Trigonella foenum-graecum demonstrated a significant zone ofinhibition against Proteus mirabilis, while Salvia hispanica and Ocimumbasilicum were particularly effective against E. coli. Coriandrum sativumexhibited broad-spectrum activity against all tested bacteria, with notable efficacy against Salmonella spp. None of the seed extracts in distilled water showed activity against the bacteria, and Coriandrum sativum did not exhibit antifungal activity against Candida spp. Antibiotic sensitivity tests with Azithromycin and Linezolid showed that the ethanol extracts were more effective than the reference drugs. The findings suggest that seed extracts could be a valuable source for developing new antimicrobial compounds
Page(s):
123-123
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025