Acetylcholinesterase Inhibitor y Activity of Venom of Hottentota tamulus (Fabricius) and Androctonus finitimus (Pocock) (Scorpiones: Buthidae) in Musca domestica | [Punjab University Journal of Zoology • 2019]

Author(s):

1. Naila Riaz: Department of Zoology, University of Sargodha,Sargodha,Pakistan

2. Hafiz Muhammad Tahir: Department of Zoology, Government College University,Lahore,Pakistan

3. Muhammad Khalid Mukhtar: Department of Zoology, University of Sargodha,Sargodha,Pakistan

4. Ali Hassan: Department of Zoology, Government College University,Lahore,Pakistan

5. Shaukat Ali: Department of Zoology, Government College University,Lahore,Pakistan

6. Shafaat Yar Khan: Department of Zoology, University of Sargodha,Sargodha,Pakistan

Abstract:

| In the present study, different venom fractions were isolated from the crude venom of Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897) by HPLC. hTe toxicity and acetylcholinesterase activity of selected venom fractions were determined using of Musca domestica adults. Only one fraction (out of 7) from H. tamulus venom and one fraction (out of 5) of A. finitimus venom were selected on the basis of their maximum yield. The approximate molecular weights of these selected venom fractions were determined by SDS Gel electrophoresis. The molecular weight of venom fractions of H. tamulus and A. finitimus used in the study were 36kDa and54kDa, respectively. The doses of venom fractions used during the study ranged from 10-30µg/ml. Both venom fractions significantly inhibited the activity of AChE. However, 30µg/ml dose of both scorpion venom caused 100% mortality in adult M. domestica. . It was concluded that the venom of scorpions contains peptides fractions that may be used as potential bio-pesticides.

Page(s): 207-211

DOI: 10.17582/journal.pujz/2019.34.2.207.211

Published: Journal: Punjab University Journal of Zoology, Volume: 34, Issue: 2, Year: 2019

Keywords:

HPLC , Scorpions , Musca domestica , Venom

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