An amperometric biosensor of determination H2 O2 Based on Horseradish Peroxidase in Carbon Nanotubes/Ionic Liquid | [Journal of Chemical Society of Pakistan • 2012]

Author(s):

1. Jun Wan: College of Environment and safety Engineering, Key Laboratory of Eco-chemical Engineering, Ministry of Education, Qingdao University of Science and Technology, Qingdao 266042, China

2. Weina Wang: College of Environment and safety Engineering, Key Laboratory of Eco-chemical Engineering, Ministry of Education, Qingdao University of Science and Technology, Qingdao 266042, China

3. Guang Yin: College of Environment and safety Engineering, Key Laboratory of Eco-chemical Engineering, Ministry of Education, Qingdao University of Science and Technology, Qingdao 266042, China

4. Xiuju Ma: College of Environment and safety Engineering, Key Laboratory of Eco-chemical Engineering, Ministry of Education, Qingdao University of Science and Technology, Qingdao 266042, China

Abstract:

A novel amperometric biosensor for the determination of H2O2 based on horseradish peroxidase (HRP) in nanocomposite material of muti-walled carbon nanotubes/ionic liquid was explored. Cyclic voltammetry (CV) was used to characterize the performance of the biosensor. Under the optimized experimental conditions, H2O2 could be detected in a linear calibration range of 0.5 × 10-6 M ~ 6.0 ×10-6 M with a correlation coefficient of 0.9902 (n = 7), a detection limit of 1.5 ×10-7 M at 3s and the recovery ratio was of 96.2% ~ 110.8%, which indicated that the accuracy of this method is also satisfied. The modified electrodes display more excellent electrochemical performance, high sensitivity, good reproducibility, and long-term stability.

Page(s): 1426-1432

DOI: DOI not available

Published: Journal: Journal of Chemical Society of Pakistan, Volume: 34, Issue: 6, Year: 2012

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