Abstract:
The global rise of antimicrobial resistance (AMR) and biofilm associated infections is one of the most pressing public health challenges of the 21stcentury, demanding sustainable and eco-friendly alternatives to synthetic antibiotics. In this context, green biotechnology and traditional ethno medicine offer promising avenues for developing climate resilient antimicrobial strategies. This study explores the antimicrobial and antibio film potential of Salvia hians, a medicinal plant indigenous to the Hindu Kush region and long valued in local ethno pharmacology for its infection-healing properties. Aqueous and decoction extracts of S. hians were prepared and subjected to phytochemical analysis, confirming the presence of flavonoids, ter penoids, and phenolic compounds. The extracts were tested against clinically relevant multidrug-resistant (MDR)pathogens, including Staphylococcus aureus (MRSA) and Escherichia coli, using agar diffusion, broth micro dilution, and biofilm disruption assays. Microscopic and spectrophotometric analyses further elucidated the inhibitory and eradication effects on pre-formed biofilms. Results revealed that S. hiansextracts significantly inhibited bacterial growth and biofilm formation in a dose dependent manner. Decoction extracts, in particular, exhibited stronger efficacy, suggesting enhanced extraction of bioactive metabolites under heat-assisted preparation. Sub-inhibitory concentrations disrupted established biofilms by more than 60%, underscoring the extracts’ dual action of growth suppression and biofilm eradication. These findings highlight Salvia hians as a sustainable phyto biotechnological resource, offering an eco-friendly strategy to address AMR and persistent biofilm infections. By integrating indigenous knowledge with modern green biotechnology, this work contributes to climate resilient therapeutic innovations and supports the global agenda of sustainable health solutions.
Page(s):
54-54
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
MRSA
,
E coli
,
biofilm inhibition
,
Antimicrobial resistance
,
Green biotechnology
,
Salvia hians
,
climate resilience