Abstract:
A complex biological response of body tissues to any harmful stimuli, inflammation remains one of the oldest challenges in the medical history. In this study, GC-MS analysis, in-vitro and in-vivo antiinflammatory potential of Desmostachya bipinnata roots was carried out. Gross morphology and histopathological study was evaluated by considering them as a potential source of drug candidate. Plant extract was prepared with ethanol to obtain the crude extract. GC-MS analysis of D. bipinnataroots revealed Tetra decahydrocyclododeca[c]furan, 12 Z-Octadecadienoic acid, nHexadecanoic acid, Oleic Acid, cis-Vaccenic acid, Bis(2-ethylhexyl) phthalate and Dodecanoic acid phytocompounds abundantly. In-vitro and in-vivo anti-inflammatory potential was carried out by Bovine serum albumin assay and Carrageenan induced rat paw edema model respectively. Concentration dependent response (50,100, 250,500,1000 µg/ml) was observed in in-vitroBSA assay. The extract showed maximum inhibition of 63.85±1.83% at 1000 µg/ml as compared to standard drug diclofenac sodium with 70.67±1.67% inhibition at highest dose 1000 µg/ml. In-vivo antiinflammatory potential of was evaluated by using carrageen an induced rat paw edema model All treated groups showed dose dependent relation at100,200 and 400mg/kg in 1,2,3,4 and 5 hr. At end of trial, rat paws were preserved in formalin solution. Gross morphology and histopathological studies of rats paws showed restoration of keratin and epithelium layer. Hence, it is concluded that D. bipinnata roots exhibited powerful anti-inflammatory potential.
Page(s):
15-15
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Antiinflammatory
,
histopathology
,
protein denaturation
,
Desmostachya bipinnata
,
carrageenan
,
albino