The high-molecular-weight glutenin subunit composition of wheat (Triticum aestivum L:) landraces from Pakistan. | [Pakistan Journal of Botany • 2003]

Author(s):

1. Rashid Anwar: Plant Genetic Resources Institute (PGRI), National Agricultural Research Centre, Islamabad, Pakistan

2. Shahid Masood: Plant Genetic Resources Institute (PGRI), National Agricultural Research Centre, Islamabad, Pakistan

3. Mir Ajab Khan: Quaid-i-Azam University, Islamabad, Pakistan

4. S. Nasim: Plant Genetic Resources Institute (PGRI), National Agricultural Research Centre, Islamabad, Pakistan

Abstract:

The endosperm storage protein of 127 wheat landraces collected from different parts of Pakistan were fractionated by SDS polvacrylamide gel electrophoresis (SDS-PAGE) to determine their high molecular weight (HMW) glutenin subunit composition. A total of 13 different subunits were found in the present set of wheat germplasm. Of these alleles detected at all the Glu-1 loci, three belong to Glu-A1, five each to Glu B1 and Glu D1 locus. Subunit null controlled by locus Glu-A1, 17+18 by Glu-B1 and 2+12 by Glu-D1, predominantly. Different accessions possessed between three and five F1MW glutenin subunit. The frequency of alleles in the entire set of germplasm ranged from 0.79 to 80.31% which indicate a higher degree of genetic diversity. The quality score calculated according to Payne (1986) ranged between 4 and 10 in the landraces examined. The information generated in this study could be utilized to improve the bread or chapati making quality.

Page(s): 61-68

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 35, Issue: 1, Year: 2003

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