An explanation for fluctuations of icariin content in Epimedium production process | [Pakistan Journal of Pharmaceutical Sciences • 2021]

Author(s):

1. Baohua Meng: Sichuan Nursing Vocational College,Chengdu,China

2. Yuansong He: Sichuan Nursing Vocational College,Chengdu,China

3. Zheyi Wang: Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China

4. Xinqi Deng: School of Life Sciences, Beijing University of Chinese Medicine,Beijing,China

5. Chuanzhen Teng: Chengdu Kanghong Pharmaceutical Co., Ltd,Chengdu,China

6. Xiao Ke: Chengdu Kanghong Pharmaceutical Co., Ltd,Chengdu,China

7. Xiaofeng Hao: Chengdu Kanghong Pharmaceutical Co., Ltd,Chengdu,China

8. Yuqing Wang: School of Chinese Materia Medica, Beijing University of Chinese Medicine,Beijing,China

9. Yujue He: School of Chinese Materia Medica, Beijing University of Chinese Medicine,Beijing,China

10. Yuan Wang: Wang Jing Hospital of CACMS,Beijing,China

11. Chunguo Wang: Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China

Abstract:

Epimedium has beneficial effects in nourishing and building up the body and is widely used in practical production of Epimedium preparations. As one of the major active compounds in Epimedium preparations, icariin is be used as a quality control index of industrial manufacture. However, content of icariin was observed to increase to uncertain extent in pharmaceutical production, which might bring difficulties in quality control. The content fluctuation mainly occurred in high-temperature extraction process. The aim of this study is to investigate what happen to flavonolglycosides in Epimedium under heating treatment. Ultra-Performance Liquid Chromatography-Linear Ion Trap Mass Spectrometer was applied to profile the transformation rule of flavonol-glycosides in Epimedium and search for an explanation for the increase in icariin content under heating treatment. 56 compounds were found to have significantly changed and their structures were identified, among which 15 flavonol-glycosides were proposed to play a role in icariin content variation. Further studies were conducted based on 8 flavonol-glycosides standard substances to obtain more credible data. Finally, Baohuoside II, 2"-o-rhamnosylicariside II, Epimedin A1, Epimedin A, Epimedin B, Epimedin C, Baohuoside I and Anhydroicaritin were found to transform into icariin during the heating process. This study provides an evidence for the quality control study of Epimedium preparation, as well as reference for chemical researches in natural pharmacy.

Page(s): 2173-2180

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 34, Issue: 6, Year: 2021

Keywords:

Quality control , icariin , Epimedium , UPLCLTQOrbitrap MS , flavonolglycosides

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