Zinc Solubilizing Bacillus Sp. Zm20 and Bacillus Aryabhattai Zm31 Promoted the Productivity in Okra (Abelmoschus esculentus L.). | [Biologia • 2018]

Author(s):

1. ISHRAT FATIMA: Department of Soil Science, University College of Agriculture & Environmental Sciences, The Islamia University of Bahawalpur, Pakistan

2. MOAZZAM JAMIL: Department of Soil Science, University College of Agriculture & Environmental Sciences, The Islamia University of Bahawalpur, Pakistan

3. AZHAR HUSSAIN: Department of Soil Science, University College of Agriculture & Environmental Sciences, The Islamia University of Bahawalpur, Pakistan

4. Muhammad Zahid Mumtaz: Institue of Molecular Biology and Biotechnology, The University of Lahore, Defense Road Campus, Lahore Pakistan

5. Muhammad Luqman: Department of Soil Science, University College of Agriculture & Environmental Sciences, The Islamia University of Bahawalpur, Pakistan

6. SAJID HUSSAIN: Stat Key Laboratory of Rice Biology, China National Rice Research Institute Hangzhou, Zhejiang Province, P.R. China

7. Saifur Rehman Kashif: Department of Environmental Sciences, University of Veterinary & Animal Sciences, Lahore, Pakistan

8. Maqshoof Ahmad: Department of Soil Science, University College of Agriculture & Environmental Sciences, The Islamia University of Bahawalpur, Pakistan

Abstract:

Zinc solubilizing bacteria (ZSB) improve crop productivity by increasing bioavailability of zinc (Zn). A pot experiment was conducted to evaluate the effectiveness of five promising ZSB strains on the productivity of okra. The experiment was conducted using Completely Randomized Design (CRD) with four replications. Data regarding physiological, growth, and yield parameters were collected and statistically analyzed. Results showed that inoculation of ZSB strains significantly increased these attributes of okra. Inoculation of strain Bacillus sp. ZM20 followed by Bacillus aryabhattai ZM31 was significantly more effective among the tested ZSB strains. Strain Bacillus sp. ZM20 improved relative water contents up to 17%, chlorophyll a and b up to 67 and 71%, respectively, plant height up to 30%, shoot fresh weight up to 19%, shoot dry weight up to 31%, root length up to 79%, root fresh weight up to 58%, root dry weight up to 66%, number of fruits plant-1 up to 89%, fruit fresh weight up to 79%, fruit dry weight up to 78%, concentration of N up to 20%, P up to 65%, K up to 20%, and protein contents up to 20% as compared to uninoculated control. It is concluded that inoculation of ZSB strains like Bacillus sp. ZM20 and Bacillus aryabhattai ZM31 is an effective approach to improve the productivity of okra (Abelmoschus esculentus L.).

Page(s): 179-185

DOI: DOI not available

Published: Journal: Biologia, Volume: 64, Issue: 2, Year: 2018

Keywords:

Keywords are not available for this article.

References:

[1] Abaid-Ullah , M.,HassanM. N.,Jamil , M.,Brader , G.,Shah , M. K. N.,Sessitsch , A., 2015.Plant growth promoting rhizobacteria: an alternate way to improve yield and quality of wheat (Triticum aestivum),Int. J. Agri. Biol 17 51 -60

[2] Adesemoye , A. O.,Obini , M.,Ugoji , E.O., 2008.Comparison of plant growth-promotion with Pseudomonas aeruginosa and Bacillus subtilis in three vegetables,Braz. J. Microbiol. 39 423 -426

[3] Ahmad , M.,Zahir , Z. A.,Asghar , H. N.,Asghar , M., 2011.Inducing salt tolerance in mung bean through co-inoculation with rhizobia and plant growth promoting rhizobacteria (PGPR) containing 1-aminocyclopropane-1- carboxylate deaminase,Canadian J. Microbiol. 57 578 -589

[4] Ahmad , M.,Zahir , Z. A.,Nadeem , S. M.,Nazli , F.,Jamil , M.,Jamshaid , M. U., 2014.Physiological response of mung bean to Rhizobium and Pseudomonas based biofertilizers under salinity stress,Pak. J. Agric. Sci. 51 555 -562

[5] Alloway , B. J., 2009.Soil factors associated with zinc deficiency in crops and humans,Environ. Geochem. Hlth. 31 537 -548

[6] Arnon , D. I., 1949.Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris,Plant 24 1 -15

[7] Bapiri , A.,Asgharzadeh , A.,Mujallali , H.,Khavazi , K.,Pazira , E., 2012.Evaluation of zinc solubilizing potential by different strains of Fluorescent Pseudomonads,J. Appl. Sci. Environ 16 295 -298

[8] Bawa , S. H.,Badrie , N., 2016.Nutrient profile, bioactive components, and functional properties of okra (Abelmoschus esculentus (L.) Moench),In Fruits, Vegetables, and Herbs 365 -409

[9] Cakmak , I., 2008.Enrichment of cereal grains with zinc: agronomic or genetic biofortification,Plant & Soil 302 1 -17

[10] Egamberdiyeva , D., 2007.The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils,Appl 36 184 -189

[11] Estrada , G. A.,Baldani,de Oliveira , D. M.,Urquiaga , S.,Baldani , J. I., 2013.Selection of phosphate-solubilizing diazotrophic Herbaspirillum and Burkholderia strains and their effect on rice crop yield and nutrient uptake,Plant & Soil 369 115 -129

[12] Freitas , A. D. S.,Vieira , C. L.,Santos , C. E. R. S.,Stamfor , N. P.,Lyra , M. C. C. P., 2007.Characterization of isolated rhizobia of pachyrhyzus erosus cultivated in saline soil of the state of pernambuco,Brazil. Bragantia, Campinas 66 497 -504

[13] Han , X. M.,Wang , R. Q.,Liu , J.,Wang , M. C.,Zhou , J.,Guo , W.H., 2007.Effects of vegetation type on soil microbial community structure and catabolic diversity assessed by polyphasic methods in North China,J. Environ. Sci 19 1228 -1234

[14] Iqbal , M.A.,Khalid , M.,Shahzad , S.M.,Ahmad , M.,Soleman , N.,Akhtar , N., 2013.Integrated use of Rhizobium leguminosarum, plant growth promoting rhizobacteria and enriched compost for improving growth, nodulation and yield of lentil (Lens culinaris Medik,Chilean J. Agric. Res. 72 104 -110

[15] Jackson , M. L., 1973.Soil Chemical Analysis,Prentice Hall. Inc. Englewood Cliffs -

[16] Jayapandi , A.,Balkrishnan , R., 1990.Correlation analysis in bhendi [Abelmoschus esculentus (L) Moench], 38 83 -85

[17] Khalid , A.,Arshad , M.,Zahir , Z. A., 2004.Screening plant growth promoting rhizobacteria for improving growth and yield of wheat,J. Appl. Microbiol 96 473 -480

[18] Khanghahi , M. Y.,Ricciuti , P.,Allegretta , I.,Terzano , R.,Crecchio , C., 2018.Solubilization of insoluble zinc compounds by zinc solubilizing bacteria (ZSB) and optimization of their growth conditions,Environ. Sci. Pollut 25 25862 -25868

[19] Khokhar , M. K.,AgricultureCorner, 2014.Production status of major vegetables in Pakistan, their problems and suggestions, 9 -

[20] Lugtenberg , B.,Kamilova , F., 2009.Plant-growthpromoting rhizobacteria,Ann. Rev. Microbiol. 63 541 -55

[21] MacDonald, 2000.The Role of Zinc in Growth and Cell Proliferation,J. Nutr 130 1500S -1508S

[22] Mayak , S.,Tirosh , T.,Glick , B. R., 2004.Plant growth-promoting bacteria confer resistance in tomato plants to salt stress,Plant Physiol. Biochem 42 565 -572

[23] Mumtaz , M. Z.,Ahmad , M.,Jamil , M.,Hussain , T., 2017.Zinc solubilizing Bacillus spp. potential candidates for biofortification in maize,Microbiol. Res. 202 51 -60

[24] Mumtaz , M. Z.,Ahmad , M.,Jamil , M.,Asad , S. A.,Hafeez , F., 2018.Bacillus strains as potential alternate for zinc biofortification of maize grains,Int. J. Agric. Biol 20 1779 -1786

[25] Nayak , D. N.,Ladha , J. K.,Watanabe , I., 1986.The fate of marker Azospirillum lipoferum inoculated into rice and its effect on growth, yield and N2 fixation of plants studied by acetylene reduction, 15N2 feeding and 15N dilution techniques,Bio. Fertil. Soils 2 7 -14

[26] Rehman , A.,Farooq , M.,Ozturk , L.,Asif , M.,Siddique , K. H., 2018.Zinc nutrition in wheat-based cropping systems,Plant Soil 422 283 -315

[27] Richardson , A. E., 2001.Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants,Aus. J. Plant Physiol 28 897 -906

[28] Rout , G. R.,Das , P., 2009.Effect of metal toxicity on plant growth and metabolism: I. Zinc, 873 -884

[29] Saravanan , V. S.,Subramoniam , S. R.,Raj , S. A., 2004.Assessing in vitro solubilization potential of different zinc solubilizing bacterial (ZSB) isolates, 35 121 -125

[30] Sharma , A.,Johri , B. N.,Sharma , A. K.,Glick , B. R., 2003.Plant growth-promoting bacterium Pseudomonas sp. strain GRP3 influences iron acquisition in mung bean (Vigna radiata L. Wilzeck),Soil Bio. Biochem 35 887 -894

[31] Steel , R. G. D.,Torrie , J. H.,Dickey , D. A., 2007.Principles and Procedures of Statistics: A Biometrical Approach, 3rd edition,McGraw Hill Book Co -

[32] Steinshamn , H.,Thuen , E.,Bleken , M. A.,Brenoe,Ekerholt , G.,Yri , C., 2004.Utilization of nitrogen and P in an organic dairy farming system in Norway,Agric. Eco. Environ 104 509 -522

[33] Vessey , J. K., 2003.Plant growth promoting rhizobacteria as biofertilizers,Plant Soil 255 571 -586

[34] Wolf , B., 1982.The comprehensive system of leaf analysis and its use for diagnosing crop nutrient status,Comm. Soil Sci. Plant 13 1035 -1059

Citations

Downloads

Views