The Role of 418 Gut Microbiota in Small Cell Lung Cancer Progression: A Mendelian Randomisation Study | [Journal of College of Physicians and Surgeons--Pakistan : JCPSP • 2025]

Author(s):

1. Rui Gong: Department of Paediatrics, General Hospital of Ningxia Medical University,Yinchuan, Ningxia Hui Autonomous Region,China

2. Haiyang Li: Department of Radiotherapy, Binhai County People's Hospital,Yancheng, Jiangsu,China

Abstract:

Objective: To investigate the causal in uence of gut microbiota on small cell lung cancer (SCLC) progression using Mendelian randomisation (MR), providing insights into the gut-lung axis in lung cancer pathology. Study Design: Analytical study. Place and Duration of the Study:Department of Radiotherapy, Binhai County People's Hospital, Yancheng, Jiangsu, China, and Department of Paediatrics, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China, from January to May 2024. Methodology: The study used 18,340 single nucleotide polymorphisms (SNPs) as instrumental variables to analyse 418 gut microbiota varieties. The inverse variance weighted (IVW) and MR Egger s methods were applied to explore causal relationships. Sensitivity analyses, including leave-one-out tests and Cochrane's Q tests, ensured robust results. A uni-directional Mendelian randomisation (MR) analysis was conducted using summary statistics from genome-wide association studies (GWAS) provided by the MiBio-Gen and Finn-Gen consortia. Results: MR identi ed several bacterial taxonomic groups signi cantly associated with SCLC risk. Protective factors incluBdaecdteroidetes (p = 0.0154), Eubacterium ruminantium group (p = 0.0241), Barnesiella (p = 0.0015), Clostridia (p = 0.0242), Christensenellaceae (p = 0.0314), Ruminococcaceae UCG-003 (p = 0.0381), and an unknown genus in theRuminococcaceae family (p = 0.0458). Conversely, the risk factors linked to increased SCLC risk included Firmicutes (p = 0.0456), Pasteurellaceae (p = 0.0177), Eubacterium oxidoreducens group (p = 0.0188), Pasteurellales (p = 0.0177), and Alcaligenaceae (p = 0.0423). Conclusion: The study suggests a protective role of speci c gut microbiota against SCLC and identi es others that may increase the risk. The absence of heterogeneity and pleiotropy supports the causal associations, underscoring the signi cance of the gut-lung axis in SCLC and the utility of MR in cancer epidemiology.

Page(s): 60-65

DOI: 10.29271/jcpsp.2025.01.60

Published: Journal: Journal of College of Physicians and Surgeons--Pakistan : JCPSP, Volume: 35, Issue: 1, Year: 2025

Keywords:

Gut microbiota , Gut microbiota , Small cell lung cancer , Cancer epidemiology , Causal inference , Mendelian randomisation

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