Pakistan Science Abstracts
Article details & metrics
No Detail Found!!
Technical Aspects of Next Generation Sequencing (NGS); Applications of Genome, Transcriptome and MicroRNAome Sequencing Information for Clinical and Research Purposes
Author(s):
1. Muhammad Akram Tariq: Higher Education Department Punjab, Lahore; Department of Physiology & Cell Biology, University of Health Sciences, Lahore; GenoMed Research Center (GMRC), Lahore, Pakistan
Abstract:
Novel DNA sequencing techniques, referred to as "next-generation" sequencing (NGS), provide high speed and throughput by increasing the number of bases obtained per sequencing run drastically while at the same time decreasing the costs per base. NGS can produce an enormous volume of sequences with many possible applications in research. In this presentation, I would provide an overview of three emerging Next generation sequencing technologies including Illumina (MiSeq, Next-Seq), Thermofisher Scientific (Ion torrent) and BGI China (MGI Sequencers) and how these instruments are being used for genome sequencing and resequencing, targeted gene sequencing, transcriptional profiling (RNA-Seq), high-throughput survey of DNA-proteininteractions (ChIP-Seq) and methylome studies. NGS can be used for metagenomics studies as well as for the detection of sequence variations within individual genomes, clinical testing especially Non-Invasive Prenatal Testing (NIPT) for genetic syndromes and Cancer panel testing for precision medicine.
Page(s): 411-411
DOI: DOI not available
Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Proteomics , Next Generation Sequencing , Whole Genome Analysis , Transcriptomics , Big Data Analysis
References:
References are not available for this document.
Citations
Citations are not available for this document.
0

Citations

0

Downloads

44

Views