Alleviation of Oxidative Effects of Salt Stress in White Lupine (Lupinus Termis L.) Plants by Foliar Treatment with L-arginine. | [Journal of Animal and Plant sciences • 2018]

Author(s):

1. SA Akladious: Biological and Geological Sciences Department, Faculty of Education, Ain Shams University, 1575 Cairo, Egypt

2. RS Hanafy: Biological and Geological Sciences Department, Faculty of Education, Ain Shams University, 1575 Cairo, Egypt

Abstract:

Salinization is one of the most important stress factors which reduce the growth and crop productivity of plants in various climatic regions. In this greenhouse study, the effects of 2.5 mM arginine as foliar spray on growth and some chemical constituents of lupine plants irrigated with three different levels of NaCl (75, 150 and 300 mM NaCl) were evaluated. The results indicated that the plants exposed to salinity stress exhibited a significant decline in growth parameters (shoot length, root length, number of leaves, fresh and dry weights of shoots and roots) and photosynthetic pigment content. On the other hand, salt stress caused significant increase in total free amino acids and total protein contents in comparison with control plants. In addition, proline content and lipid peroxidation increased with increasing concentrations of NaCl. Treatment with 2.5 mM arginine as foliar spray mitigated salt stress by inducing enzyme activities responsible for antioxidation, e.g., superoxide dismutase, ascorbate peroxidase, glutathione reductase, detoxification as well as improving all the above recorded parameters. Furthermore, treating the plants with 2.5 mM arginine alone or in combination with salt stress leads to differential expression of the genetic information in lupine plants, resulting in changes in gene products, including protein and isozymes profiles. These changes induced the synthesis of certain proteins and simultaneously decreased the expression of other protein sets. These findings confirm the effectiveness of spraying lupine plants with arginine on alleviating salinity stress in lupine plants.

Page(s): 165-176

DOI: DOI not available

Published: Journal: Journal of Animal and Plant sciences, Volume: 28, Issue: 1, Year: 2018

Keywords:

antioxidant enzymes , salinity , Amino acids , Arginine

References:

[1] AbdEl-Monem,A.A.,,2007,Polyamines as modulators of wheat growth, metabolism and reproductive development under high temperature stress,Ph.D. thesis. Ain Shams Univ., Cairo,,,

[2] Abdul-Qados , A.M.S.,,2010,Effect of arginine on growth, nutrient composition, yield and nutritional value of mung bean plants grown under salinity stress,Natu. & Sci. 8,,30,42

[3] Akladious , S. A,S. M.Abbas,,2014,Inter simple sequence repeat (ISSR) markers and some physiological attributes of barley (Hordeum vulgare L.) genotypes to drought and potassium nutrition,The J. Anim. Plant Sci,24,620,633

[4] Astorga , G. I. A.,L. A.Meléndez,,2010,Salinity effects on protein content, lipid peroxidation, pigments, and proline in Paulownia imperialis (Siebold & Zuccarini) and Paulownia fortunei (Seemann & Hemsley) grown in vitro,Electro. J. Biotech,13,1,15

[5] Azevedo-Neto , A.D.,J.T.Prisco,J.Enéas-Filho,C.E.B.Abreu,E.Gomes-Filho,,2006,Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt sensitive maize genotypes,Environ. Exp. Bot,56,87,

[6] Barand , A.,F.Nasibi,K.H.Manouchehri Kalantari,,2015,The effect of arginine pretreatment in the increase of cold tolerance in Pistacia vera L. in vitro,Russ. Agric. Sci,41,340,

[7] Bates , L.S.,R.P.Waldren,L.D.Teare,,1973,Rapid determination of free proline for water stress studies,Plant and Soil,39,205,

[8] Beauchamp , C.,and I. Fridovich,,1971,Superoxide dismutase: improved assays and an assay applicable to acrylamide gel,Anal. Bio. Chem,44,276,

[9] Bekheta , M.A.,H.M.S.El-Bassiouny,,2005,Response of two wheat cultivars grown under salinity stress to putrescine treatment,J. Agric. Sci. Mansoura,30,4505,4521

[10] Brauc , S.,E.D.Vooght,J.M.C. Geuns , M.,Höfte , and G.,Angenon,,2012,Overexpression of arginase in Arabidopsis thaliana influences defense responses against Botrytis cinerea,Plant Biol,14,39,

[11] Coueen , I.,C.Sulmon,G.,A . El-Amrani,,2004,Involvement of polyamines in root development,Netherlands,76,1,10

[12] da Rocha , I.M.A.,V.A.Vitorello,J.S.Silva,S.L.Ferreira-Silva,R.A.Viégas,E.N.Silva,,2012,Exogenous ornithine is an effective precursor and the ´-ornithine amino transferase pathway contributes to proline accumulation under high N recycling in salt-stressed cashew leaves,J. Plant. Physiol,169,41,

[13] Dolatabadian , A.,R.J.Saleh Jouneghani,,2009,Impact of exogenous ascorbic acid on antioxidant activity and some physiological traits of common bean subjected to salinity stress,Not. Bot. Hort. Agrobot. Cluj,37,165,172

[14] El-Bassiouny , H.M.S.,H.A.Mostafa,S.A.El,Khawas,R.A.Hassanein,S.I.Khalil,A.A.Abd El Monem,,2008,Physiological responses of wheat plant to foliar treatments with arginine or putrescine,Aust. J. Basic. Appl. Sci. 2,,1390,1403

[15] El-Baz , F. K.,A. A.Mohamed,A. A.Aly,,2003,Development of biochemical markers for salt stress tolerance in cucumber plants,Pakistan J. Bio. Sci. 6,,16,

[16] FAO,,2009,Food and Agriculture Organization of United Nations, Agric Data FAO,,,,

[17] Foyer , C.H.,Halliwell,,1976,The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism,Planta,133,21,25

[18] Heath , R.L.,Packer,,1968,Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation,Arch. Bioch. Bioph,125,189,

[19] Hozayn , M.,A.A.Abd,M.Ebtihal,E.M.Abd,M.S.A.Amira,,2013,Amelioration of salinity stress in mungbean (Vigna radiate L). plant by soaking in arginine,J. Appl. Sci. Res,9,393,401

[20] Khalil , S.I.,H.M.El-Bassiouny,R.A.Hassanein,H.A.Mostafa,S.A.El-Khawas,A.A.Abd ElMonem,,2009,Antioxidant defense system in heat shocked wheat plants previously treated with arginine or putrescine,Aust. J. basic appl. Sci,3,1517,1526

[21] Laemmli , U.K.,,1970,Cleavage of structural proteins during assembly of head bacteriophage T4,Nature,227,680,

[22] Larsen , A.L.,Benson,,1970,Variety specific variants of oxidative enzymes from soybean seeds,Crop Sci,10,493,

[23] Lichtenthaler , H.K.,,1987,Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes,Methods Enzymol,148,350,382

[24] Lowry , O.H.,N.J.Rosembrough,A.L.Farr,R.J . Randall,,1951,Protein measurement with the Folin phenol reagent,J Biol. Chem,193,267,275

[25] Lu , X.,X.Zhao,D.Wang,Z.Yin,J.Wang,W.Fan,S.H.Wang,T.Zhang,W.Ye,,2015,Wholegenome DNA methylation analysis in cotton (Gossypium hirsutum L.) under different salt stresses,Turk. J. Biol,39,396,

[26] Manai , J., T.,Kalai , H.,Gouia , and F.J.,Corpas,,2014,Exogenous nitric oxide (NO) ameliorates salinity-induced oxidate stress in tomato (Solanum lycopersicum) plants,J Soil Sci and Plant Nutr,14,433,446

[27] Mohamed , A. A.,,2005,Two-dimensional electrophoresis of soluble proteins and profile of some isozymes isolated from maize plant in response to NaCl,Res. J. Agri. Bio. Sci. 1,,38,

[28] Mohammadkhani , N.,R.Heidari,N.Abbaspour,F.Rahmani,,2016,Salinity effects on expression of some important genes in sensitive and tolerant grape genotypes,Turk. J. Biol,40,95,108

[29] Moore , S.,W.H.Stein,,1954,A modified ninhydrin reagent for the photometric determination of amino acids and related compounds,J. Biol. Chem,211,907,913

[30] Mostafa , H.A.M.,R.A.Hassanein,S.I.Khalil,S.A.ElKhawas , H.M.S. El-Bassiouny ,A.A. AbdEl-Monem,,2010,Effect of arginine or putrescine on growth, yield and yield components of late sowing wheat,Inter. J. Appl. Sci. Res,6,177,183

[31] Nakano , Y.,K.Asada,,1981,Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplast,Plant Cell Physiol,22,867,880

[32] Nasibi , F.,K.K.H. Manouchehri,A . Barand,,2014,Effects of seed pre-treatment with L-arginine on improvement of seedling growth and alleviation of oxidative damage in canola plants subjected to salt stress,IJPP,5,1217,1224

[33] Nasibi , F.,M.Yaghoobi,K.H.Manouchehri,,2011,Effect of exogenous arginine on alleviation of oxidative damage in tomato plant under water stress,J. Plant Interact,6,291,296

[34] Nasri , N., S.,Maatallah , I. Saidi,Lachaâl,Anim,,2017,Influence of salinity on germination, seedling growth, ion content and acid phosphatase activities of Linum usitatissimum L. The J,Plant Sci,27,517,521

[35] Nejadalimoradi , H.,F.Nasibi,K.M. Kalantari , and R.,Zanganeh,,2014,Effect of seed priming with Larginine and sodium nitroprusside on some physiological parameters and antioxidant enzymes of sunflower plants exposed to salt stress,Agric. Communi,2,23,30

[36] Pal , M.,G.Szalai,T.Janda,,2015,Polyamines are important in abiotic stress signaling,Plant Sci,237,16,23

[37] Priya , A. M.,S. R.Krishnan,Ramesh,,2015,Ploidy stability of Oryza sativa. L cv IR64 transformed with the moth bean P5CS gene with significant tolerance against drought and salinity,Turk. J. Biol,39,407,

[38] Rady , M.M.,M.Sadak,H.M.S.El-Bassiouny,A.A. AbdEl-Monem,,2011,Alleviation the adverse effects of salinity stress in sunflower cultivars using nicotinamide and ±-tocopherol,Aust. J. Basic Appl. Sci,5,342,355

[39] Rasul , I.,H.Nadeem,M.H.Siddique,R.M.Atif,M.A.Ali,A.Umer,F.Rashid,M.Afzal,M.Abid,F.Azeem,,2017,Plants sensory-response mechanisms for salinity and heat stress,The J. Anim. Plant Sci,27,490,502

[40] Sadak , M. S.,A. A.Abd,H. M. S. ElBassiouny,N. M.Badr,,2012,Physiological response of sunflower (Helianthus annuus L.) to exogenous arginine and putrescine treatments under salinity stress,J. Appl. Sci. Res,8,4943,4957

[41] Saqib , Z. A.,J.Akhtar,M. A.Ul-Haq,and I. Ahmad,,2012,Salt induced changes in leaf phenology of wheat plants are regulated by accumulation and distribution pattern of Na+ ion,Pakistan J. Agri. Sci,49,141,148

[42] SAS Programme,,1982,SAS user sguide statistics (computer program),SAS Institute,,584,

[43] Sato , M.,M.Hasegawa,,1976,The latency of spinach chloroplast phenolase,Phytochem,15,61,65

[44] Stegemann , H.,A.M.R.Afifiy,K.R.F.Hussein,,1985,Cultivar Identification of dates (Phoenix dectylifera) by protein patterns. 2nd international Symposium of Biochemical Approaches to Identification of Cultivars,,,,

[45] Wiatr , K.,A.Dolata,T. MaDczak,,2007,Concentration and variation of basic quality traits of seeds of lupine cultivars registered in Poland,Zesz. Prob. Post Nauk. Rol,522,75,85

[46] Zeid , M.,,2004,Response of bean (Phaseolus vulgaris) to exogenous putrescine treatment under salinity stress,Pakistan J. Biol. Sci,7,215,229

[47] Zhang , S.,H.Jiang,S.Peng,H.Korpelainen,C.Li,,2011,Sex-related differences in morphological, physiological and ultrastructural responses of Populus cathayana to chilling,J. Exp. Bot,62,675,

Citations

Downloads

Views