Prevalence of Hepatitis B Virus in Southern Punjab, Pakistan using Real Time PCR | [Abstract Book on Global Science Technology and Management Conference • 2023]

Author(s):

1. Ghazala Shaheen: Faculty of Veterinary and Animal Sciences, Department of Microbiology, The Islamia University, Bahawalpur, Pakistan

2. Naila Irshad: Faculty of Life Sciences, Department of Microbiology and Molecular Genetics, The Women University Multan, Pakistan

3. Mudassar Mohiuddin: Faculty of Veterinary and Animal Sciences, Department of Microbiology, The Islamia University, Bahawalpur, Pakistan

4. Atia Iqbal: Faculty of Life Sciences, Department of Microbiology and Molecular Genetics, The Women University Multan, Pakistan

5. Manahil Khanam: Faculty of Life Sciences, Department of Microbiology and Molecular Genetics, The Women University Multan, Pakistan

6. Hira Hameed: Faculty of Veterinary and Animal Sciences, Department of Microbiology, The Islamia University, Bahawalpur, Pakistan

7. Hafiz Muhammad Imran: Faculty of Veterinary and Animal Sciences, Department of Animal Nutrition, The Islamia University, Bahawalpur, Pakistan

Abstract:

Hepatitis B virus (HBV) is a main causative agent of severe, early and prolonged liver infections that result in cirrhosis of the liver and hepatocellular cancer. HBV infection is a global health issue, with a growing burden on developing countries such as Pakistan. This study was conducted to determine the prevalence of hepatic infection, viz, hepatitis B (HBV) virus, in the population of Southern Punjab, Pakistan, with respect to their gender, high and low viral load, and marital status. A total of 176 HBV samples of seropositive patients (124 male and 52 female) were sent to MINAR (PAEC Cancer hospital Multan, Pakistan), having a mean age of 36.5 ±14.15 years) to detect the prevalence of the Hepatitis B virus (HBV) using Real-Time PCR. Chi-square analysis was applied on pad prism to calculate statistically significant results. Out of the total of 176 subjects, 144(81.8%) were HBV DNA PCR positive, and 32(18.2%) were HBV DNA PCR negative. The percentage of HBV in males (84.68%) was significantly higher as compared to females (26.9%, P<0.05). High viral load was not significantly different in males (3.2%) and females (5.7%) (P= 0.4), and the same was observed in the case of low viral load, males (18.5%) and females (9.61%) (P=0.2). The incidence of HBV in married people (89%) was significantly higher as compared to single persons (57%) (P < 0.05). The prevalence of HBV infection was higher in males as compared to females in the studied population. The viral load had no difference in both genders (males & females). The high level of HBV infection in the studied population is of concern, indicating the need for implementing preventive measures. The frequency of HBV incidence in married patients was higher than in single persons.

Page(s): 6-6

DOI: DOI not available

Published: Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023

Keywords:

Polymerase Chain Reaction , Hepatitis B virus , Southern Punjab , Real Time PCR , viral load

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views