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A simple method for producing aflatoxin b1 on rice medium for use in experimental animal feeds
Author(s):
1. A. W. Yunus: Institute of Animal Nutrition, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine Vienna, A-1210 Vienna, Austria
2. J. Böhm: Institute of Animal Nutrition, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine Vienna, A-1210 Vienna, Austria
Abstract:
A simplification and standardisation of the existing methods to produce Aflatoxin B1 (AFB1) on rice medium is described. As per conventional method, rice were first soaked in 1 ml water/2 g of rice for 2 hr, autoclaved, and then inoculated with Aspergillus flavus. During different stages of incubation, water was added according to different regimens. It was concluded that 1 ml water/100 g of rice at 24 to 36 hr of incubation is the maximum amount of water that could be added for optimal AFB1 production. 
Page(s): 303-304
DOI: DOI not available
Published: Journal: Journal of Animal and Plant sciences, Volume: 21, Issue: 2, Year: 2011
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