The isolation of pre-adipocytes from dairy cow adipose tissue and the development of preadipocytes into mature adipocytes. | [Pakistan Veterinary Journal • 2015]

Author(s):

1. Liheng Yin: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

2. Xia Qin: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

3. Qinghua Deng: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

4. Yuming Zhang: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

5. Lin Lei: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

6. Wenwen Gao: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

7. Zhicheng Peng: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

8. Xiliang Du: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

9. Guowen Liu: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

10. Xinwei Li: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xi’an Road, Changchun, Jilin, China

Abstract:

Serious lipid mobilization in adipose tissue of dairy cow is the pathological basis of ketosis and fatty liver. Many Studies demonstrated that pre-adipocytes are unstable and can differentiate into multiple cells in vitro. The aim of this study was to investigate the differentiation process of dairy cow pre-adipocytes and to establish a stable pre-adipocyte induction method. Pre-adipocytes were isolated from dairy cow adipose tissue and were cultured for 15 days. The originally rounded cells converted to a spindle fibroblast-like morphology and accumulated lipids during culturing. The lipid droplets and TG content in the adipocytes gradually increased from 4 to 15 days in culture. The adipocytes reached maximal proliferation after 11 days in culture. Additionally, the expression of peroxisome proliferator-activated receptor γ2 (PPARγ2) and sterol regulatory element binding protein 1c (SREBP-1c) increased during the initial 4 days of differentiation and decreased thereafter. However, the level of SREBP-1c increased after 10 days of differentiation. The PPARγ2 and SREBP-1c proteins levels were significantly higher on day 13 than on day 0. PPARγ2 and SREBP-1c were primarily localized to the cytoplasm on day 0 and to the cytoplasm and nucleus on day 13. In conclusion, a stable dairy cow preadipocyte culture was established, and SREBP-1c and PPARγ2 were increased during (demonstrated to be involved in) pre-adipocyte differentiation. Isolated adipocytes can be used as a cellular model to elucidate the process of lipogenesis in dairy cows and to further investigate metabolic diseases.

Page(s): 283-288

DOI: DOI not available

Published: Journal: Pakistan Veterinary Journal, Volume: 35, Issue: 3, Year: 2015

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