Abstract:
Introduction: Nigella sativa (black seeds) is an herbal product belongs to ranuncul aceae family which is an annual herbaceous medicinal plant has oldest historical and religious background. Objective: To evaluate the effect of various doses of Nigella sativa in Isoniazid-induced liver biochemical changes in Rabbits. Study design: Animal Study (randomized case-control). Place of study: Department of Pharmacology and Therapeutics in collaboration with the Animal House BMSI, JPMC Karachi. Methods: In this animal study, 48 healthy rabbits (n=8) of either sex were randomly selected and equally divided into four groups (n=12 in each group). Group I was given healthy diet only. Group II was given Isoniazid at 50mg/kg body weight per day orally. Group III was given Isoniazid 50mg/kg plus Nigella sativa 500 mg/kg body weight per day orally and Group IV was given Isoniazid 50mg/kg plus Nigella sativa 1gm/kg body weight per day orally. The study duration was 20 days. Liver biochemical changes, i.e. Serum Total Bilirubin, Alanine Transaminase (ALT) and Aspartate Transaminase (AST), were investigated on 00 and 21 days. The data was collected using a design proforma and assessed statistically with SPSS version 16.0. Paired T-Test was applied. Results: In Group I, the liver biochemical changes were non-significant on day 00 and 21 (S. Total Bilirubin, p =0.06; ALT, p=0.09 & AST, p=0.74). In Group II, the parameters were significantly raised, when compared on day 00 and 21 (S. Total Bilirubin, p=0.00; ALT, p=0.00 & AST, p=0.00). In Group III, the liver biochemical changes noted were both significant and non-significant when compared on day 00 and 21 (S. Total Bilirubin, p=0.06; ALT, p=0.00 & AST, p=0.74). In Group IV, the liver biochemical changes were non-significant when compared on day 00 and 21 (S. Total Bilirubin, p=0.06; ALT, p=0.09 & AST, p=0.74). Conclusion: Nigella sativa showed a protective role in isoniazid-induced liver toxicity in rabbits.
Keywords:
Aspartate transaminase AST
,
Alanine transaminase ALT
,
Tuberculosis TB
,
Isoniazid INH
,
Antituberculosis Therapy ATT
,
Nigella sativa NS
,
Serum total bilirubin