Abstract:
ATZ (ATZ) is a selective herbicide of the triazine group which is widely used for weed control, commonly in maize,sugarcane and nuts. Propolis is a sticky, resinous material that is used by bees to maintain structure of the hives. Bee propolis, has several medicinal value like antibacterial, anticancer, bacteriostatic. This study evaluated the protective effect of bee propolis on genotoxicity against ATZ intoxication in grass carp (Ctenopharyngodon idella). The 96 hours LC50 value was calculated to be 15mg/L at 30 °C. Forty fish were equally divided into two main groups (n= 20 fish per group) for 25 days. The first group was control group which was fed on control commercial diet. Second group was fed on propolis prepared diet. After this initial period, both groups were further divided into four subgroups two subgroups was control diet (C1 & C2) and two subgroups was propolis prepared diet groups (P1 & P2). Among the four subgroups, C1 and P1 were left untreated, while the other two subgroups, C2 and P2, were treated with the calculated LC50 value of atrazine for four consecutive days. The genotoxicity was assessed through two different tests (Micronucleus (MN) & DNA fragmentation). The results of the MN test showed that the mean values of genotoxicity indicators in the control diet subgroups were 5.60 (C1) and 46.77 (C2), whereas in the propolis diet subgroups, they were 3.90 (P1) and 29.72 (P2). In the DNA fragmentation test, the genotoxicity indicators exhibited values of 57 (C1), 120.4 (C2), 42.60 (P1), and 80 (P2). Overall, the findings of this study demonstrate the potential protective effect of bee propolis against genotoxicity induced by atrazine intoxication in grass carp. The propolis diet showed a significant reduction in genotoxicity indicators compared to the control diet, suggesting its beneficial impact in minimizing the harmful effects of atrazine. Further investigations are warranted to elucidate the underlying mechanisms and to explore the potential application of bee propolis in mitigating genotoxicity in aquatic organisms exposed to environmental contaminants.
Page(s):
322-322
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Grass carp
,
LC50 value
,
Bee propolis
,
Genotoxicity
,
atrazine intoxication