The influence of taurine supplementation in lactose egg yolk glycerol extender for cryopreservation of buffalo bull (Bubalus bubalis) semen. | [Journal of Animal and Plant sciences • 2013]

Author(s):

1. D. H. Mughal: Department of Physiology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

2. A. Ijaz: Department of Physiology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

3. M. S. Yousaf: Department of Physiology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

4. H. Rehman: Department of Physiology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

5. M. Aleem: Department of Theriogenology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

6. H. Zaneb: Department of Anatomy and Histology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

7. I. Rabbani: Department of Physiology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

8. F. Wadood: Department of Theriogenology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

Abstract:

The objective of this study was to assess the influence of supplementing various concentrations of taurine in lactose egg yolk glycerol extender (LEGE) on spermatozoa characteristics. Ejaculates of four routinely used Nili- Ravi buffalo bulls were collected once in a week for five weeks using an artificial vagina at Semen Production Unit, Qadirabad, Sahiwal, Pakistan. Pooled semen samples were diluted at 37°C in LEGE extender containing taurine (TA) (0.0, 20.0, 40.0, 60.0 mM) and aspirated into French straws (0.5 ml) having 20 × 106 spermatozoa. Filled and sealed semen containing straws were cooled from 37 to 4°C in a cold cabinet for 4 h before placing it 4 cm above liquid nitrogen (LN) vapors for 20 minutes and stored in LN. These stored straws were thawed individually at 37°C for 30 s in a water bath for evaluation of spermatozoa characteristics such as motility, viability, acrosomal/plasma membrane/DNA integrity and lipid peroxidation. The results indicate that spermatozoa motility (%) significantly decreased (P0.05) among control (TA 0.0), TA 20.0 and TA 40.0 mM supplemented groups. Similarly, spermatozoa DNA integrity rate (%) was significantly decreased (P

Page(s): 715-720

DOI: DOI not available

Published: Journal: Journal of Animal and Plant sciences, Volume: 23, Issue: 3, Year: 2013

Keywords:

Keywords are not available for this article.

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views