Validation and application of high performance liquid chromatographic method for the estimation of metoclopramide hydrochloride in plasma | [Pakistan Journal of Pharmaceutical Sciences • 2017]

Author(s):

1. Ahmad Khan: Department of Pharmacy, Quid-i-Azam University,Islamabad,Pakistan

2. Jallat Khan: Department of Chemistry, Islamia University,Bahawalpur,Pakistan

3. Muhammad Irfan: Deparment of Pharmacy, GC University,Faisalabad,Pakistan

4. Syed Baqir Shyum Naqvi: Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University,Karachi,Pakistan

5. Gul Majid Khan: Department of Pharmacy, Quid-i-Azam University,Islamabad,Pakistan

6. Muhammad Harris Shoaib: Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi,Karachi,Pakistan

7. Rabia Ismail Yousaf: Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi,Karachi,Pakistan

8. Amjad Khan: Department of Biotechnology, Quid-i-Azam University,Islamabad,Pakistan

Abstract:

The objective of this study was validation of a reverse phase HPLC method for the estimation of metoclopramide HCl in plasma already validated for determination of metoclopramide HCl in tablets dosage form. A reverse chromatographic method was used for estimation of metoclopramide HCl with the mobile phase of acetonitrile, 20mM potassium dihydrogen phosphate buffer solution (pH 3.0 adjusted with orthophosphoric acid) in the ratio of 40: 60. The column used was Waters C18 3.9×300mm µBondapak (RP). The flow rate of the mobile phase was 2ml/ minute. The detector was set at the wavelength of 275nm. This method validated in plasma and was found to be linear, with correlation coefficient (R2), value of 0.9988, in the range of 48 ng/ml-0.25ng/ml. The method modified was accurate, precise, sensitive and showed good stability results. The % RSD of the retention time and peak area of metoclopramide HCl was 0.19% and 1.44% respectively. All the parameters such as specificity, linearity, range, accuracy, precision, system suitability, solution stability, detection and quantification limits were evaluated to validate this method and were found within the acceptance limits. The method can be effectively used for estimation of metoclopramide HCl in plasma.

Page(s): 143-147

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 30, Issue: 1, Year: 2017

Keywords:

Pharmacokinetics , HPLC , plasma , Validation , Metoclopramide HCl

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