The expression and role of caveolin-1 in different development periods of Drosophila melanogaster. | [Pakistan Journal of Zoology • 2016]

Author(s):

1. Yejun Zhang: School of Life Sciences, Liaoning Normal University, Dalian, China; Liaoning Key Lab of Biotechnology and Molecular Medicine Development, Dalian, China

2. Ying Wang: School of Life Sciences, Liaoning Normal University, Dalian, China

3. Changqing Qu: School of Life Sciences, Liaoning Normal University, Dalian, China

4. Hui Huang: School of Life Sciences, Liaoning Normal University, Dalian, China

5. Hongyan Li: School of Life Sciences, Liaoning Normal University, Dalian, China; Liaoning Key Lab of Biotechnology and Molecular Medicine Development, Dalian, China

6. Yan Li: School of Life Sciences, Liaoning Normal University, Dalian, China

7. Yuying Cui: School of Life Sciences, Liaoning Normal University, Dalian, China

8. Wei Zou: School of Life Sciences, Liaoning Normal University, Dalian, China; Liaoning Key Lab of Biotechnology and Molecular Medicine Development, Dalian, China

Abstract:

Caveolin-1 is an iconic structural molecule of Caveolae. Because of its pivotal role in the process of cell proliferation, apoptosis and other related signaling pathways of human tumors, caveolin-1 has been extensively studied. It has been found that caveolin-1 expresses in various types of invertebrates and vertebrates and is highly conserved in evolution. However, there has been no report about the caveolin-1 of insect so far. In the present study, caveolin-1 was amplified in the larvae and adults of Drosophila melanogaster to observe their expression levels. The in vivo distributions of caveolin-1 were observed in larvae, pupae and adults through immunofluorescence and confocal technology. Furthermore, the expression of caveolin-1 was detected in larvae, pupae and adults through Western blotting. The results showed that caveolin-1 exists in D. melanogaster, and its expression and distribution varied in different developmental stages. The in vivo distribution of caveolin-1 was mainly in the brain, muscles and gut wall in adult, a little in the brain and fat bodies in pupa and a little in the fat bodies in larva. The expression levels of caveolin-1 were significantly higher than in adults in the larvae and pupae. The expression of the protein was significantly higher in the female adult than in the adult male.

Page(s): 1255-1261

DOI: DOI not available

Published: Journal: Pakistan Journal of Zoology, Volume: 48, Issue: 5, Year: 2016

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