Abstract:
Failed hemostasis leads to the formation of undesirable blood clots in the blood vessels leading to the condition called thrombosis, which is a primary cause of deaths among cardiovascular diseases world-wide. Conventional thrombolytic agents such as streptokinase, tissue plasminogen activator, urokinase, has several limitations such as higher cost of production as well as their side effects also forced to find alternative and safer fibrinolytic enzymes. Thrombolytic agents are administered to liquefy the blood lump. Fibrinolytic enzymes with a direct mechanism of action and safer properties are currently requested for thrombolytic therapy. The current research work aimed at optimization, production, purification and evaluation of fibrinolytic enzyme isolated from Bacillus subtilis. Indigenous soil samples were collected. After processing, samples were inoculated on Nutrient Agar Media Plates and Fermentation Media was used for extraction of Bacillus subtilis. Cotton seed cake was used as substrate for the isolated strain. Isolated strain of Bacillus subtilis was used for the production and purification of Fibrinolytic Enzyme. The enzyme (Fibrinolytic) was purified by ion exchange column chromatography, rather the protein was characterized by using SDS-PAGE. To seek the optimum activity, different thermodynamic parameters like pH, temperature and molecular weight were applied on the purified enzyme. The purified enzyme from (isolated strain) showed strong and optimal production of fibrinolytic activity with 82.99 U/mg specific activity, 298 U/ml enzyme activity, 3.6 mg/ml protein, 6.17-fold purification and 80.74% recovery while the molecular weight of the enzyme was approximately 29kDa. Fibrinolytic enzyme produced from the isolated strain exhibited activity within the pH range of 7 to 8 while it gave its best performance at pH 7. Thermal stability between 40°C to 60°C was shown by the fibrinolytic enzyme. Bacillus subtilis growth was improved for this purpose utilizing various optimized factors (pH, temperature, substrate concentration and fermentation duration). Bacillus subtilis would develop to its greatest potential under these optimum conditions, permitting for the industrial production of high-quality fibrinolytic enzyme at a minimal cost for the treatment of cardiovascular diseases. In recent work we have made an attempt to present the importance of Bacillus spp. as a source of fibrinolytic enzyme.
Page(s):
90-90
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Bacillus subtilis
,
cardiovascular disorder
,
fibrinolytic enzyme
,
Thrombolytic agent