The Protective Effects of Boric Acid against Acrylamide-induced Pulmonary Damage in Rats | [Pakistan Veterinary Journal • 2024]

Author(s):

1. Suzan Onur: Faculty of Health Science, Karabuk University,Karabuk,Türkiye

2. Mustafa Cengiz: Department of Elementary Education, Faculty of Education, Siirt University,Siirt,Türkiye

3. Adnan Ayhanci: Department of Biology, Faculty of Science, Eskişehir Osmangazi University,Eskişehir,Türkiye

Abstract:

The purpose of this study was to investigate whether boric acid (BA) can reduce the acute lung injury caused by acrylamide (ACR) in rats. ACR was orally given to produce acute lung damage. Group 1 (saline), Group 2 (ACR, 38.27 mg/kg)), Group 3 (20 mg/kg BA), Group 4 (10 mg/kg BA+ACR), and Group 5 (20 mg/kg BA+ACR) were five equal groups of thirty rats. Lungs were taken for histological analysis, immunohistochemical staining and biochemical evaluations after the experimental trial. Malondialdehyde (MDA) levels, a marker of lipid peroxidation and oxidative stress, increased after ACR administration. Nevertheless, it caused the reduction of glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) in lungs. The administration of BA efficiently inhibited the activities of CAT, GSH, SOD, and GPx in lung tissue and protected the lipid peroxidation caused by ACR. Moreover, increased expression of Bax, Caspase-3 and reduced Bcl-2 were found in the lung tissue of rats given ACR, which indicated enhanced congestion, inflammation, alveolar damage, and apoptosis. Interestingly, in rats given ACR injections, BA enhanced the expression of indicators of histological damage and lung injury. These results indicate the efficacy of BA treatment in preventing lipid peroxidation caused by ACR and reducing the negative effects on antioxidant capacity.

Page(s): 949-953

DOI: 10.29261/pakvetj/2024.229

Published: Journal: Pakistan Veterinary Journal, Volume: 44, Issue: 3, Year: 2024

Keywords:

Acrylamide Antioxidant Biochemical toxicity Boric acid Pulmonary damage

References:

[1] ALKarim S,ElAssouli S,Ali S .2015 .Effects of low dose acrylamide on the rat reproductive organs structure, fertility, and gene integrity. Asian Pac J Reprod, 4 : 179-187.

[2] Ayhanci A,Tanriverdi DT,Sahinturk V .2020 .Protective effects of boron on cyclophosphamide-induced bladder damage and oxidative stress in rats. Biol Trace Elem Res, 197 : 184-191.

[3] Batoryna M,Semla-Kurzawa M,Zyśk B .2019 .Acrylamide-induced alterations in lungs of mice in relation to oxidative stress indicators. J Environ Sci Heal B, 54 : 745-751.

[4] Becalski A,Lau BPY,Lewis D,Seaman SW,Modeling SW .2003 .. J Agric Food Chem, 51 : 802-808.

[5] Cengiz M .2018 .Boric acid protects against cyclophosphamide-induced oxidative stress and renal damage in rats. Cell Mol Biol, 64 : 11-14.

[6] Cengiz M,Ayhanci A,Akkemik E .2022 .The role of Bax/Bcl-2 and Nrf2-Keap-1 signaling pathways in mediating the protective effect of boric acid on acrylamide-induced acute liver injury in rats. Life Sci, 307 : 120864.

[7] Cengiz M,Cetik Yildiz S,Demir C .2019 .Hepato-preventive and anti-apoptotic role of boric acid against liver injury induced by cyclophosphamide. J Trace Elemen Med Biol, 53 : 1-7.

[8] Chen JH,Yang CH,Wang YS .2013 .Acrylamide-induced mitochondria collapse and apoptosis in human astrocytoma cells. Food Chem Toxicol, 51 : 446-452.

[9] Elsayed A,Aboubakr M,Hassan FW .2024 .Testicular injury of acrylamide in rats and potential protection of coenzyme Q10 and rosuvastatin. Pak Vet, : .

[10] Exon J .2006 .A review of the toxicology of acrylamide. J Toxicol Environ Heal B, 9 : 397-412.

[11] Friedman M .2003 .. J Agric Food Chem, 51 : 4504-4526.

[12] Gür F,Cengiz M,Gür B,Cengiz O .2023 .Therapeutic role of boron on acrylamide-induced nephrotoxicity, cardiotoxicity, neurotoxicity, and testicular toxicity in rats: Effects on Nrf2/Keap1 signaling pathway and oxidative stress. J Trace Elemen Med Biol, 80 : 127274.

[13] Ince S,Keles H,Erdogan M .2012 .Protective effect of boric acid against carbon tetrachloride-induced hepatotoxicity in mice. Drug Chem Toxicol, 35 : 285-292.

[14] Jabbar AA,Alamri ZZ,Abdulla MA .2024 .Boric acid (Boron) attenuates AOM-induced colorectal cancer in rats by augmentation of apoptotic and antioxidant mechanisms. Biol Trace Elem Res, 202 : 2702-2719.

[15] Jiang G,Lei A,Chen Y .2021 .The protective effects of the Ganoderma atrum polysaccharide against acrylamide-induced inflammation and oxidative damage in rats. Food Funct, 12 : 397-407.

[16] Uthra C,Shrivastava S,Jaswal A .2017 .Therapeutic potential of quercetin against acrylamide induced toxicity in rats. Biomed Pharmacother, 86 : 705-714.

[17] Wang B,Wang X,Yang S .2021 .Acrylamide exposure and pulmonary function reduction in general population: The mediating effect of systemic inflammation. Sci Total Environ, 778 : 146304.

[18] Yamada KE,Eckhert CD .2019 .Enhances Antioxidant Status. Biol Trace Elem Res, 188 : 2-10.

[19] Yesildag K,Eroz R,Genc A .2022 .Evaluation of the protective effects of morin against acrylamide-induced lung toxicity by biomarkers of oxidative stress, inflammation, apoptosis, and autophagy. J Food Biochem, 46 : e14111.

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