An improved HPLC method for the determination of Ethionamide in serum. | [Journal of Chemical Society of Pakistan • 2008]

Author(s):

1. Asad Ullah Madni: Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

2. Mahmood Ahmad: Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

3. Naveed Akhtar: Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

4. Mohammad Ashraf: Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan

5. Zeba Ahmad Shuja: The Schazoo Laboratories, Lahore, Pakistan

Abstract:

simple and sensitive HPLC method was developed for the determination of ethionamide (ETA) in serum. The method was based on liquid liquid extraction with acetonitrile causing precipitation of serum proteins, drying of supernatant layer and reconstitution with mobile phase. The separation was done by reverse phase chromatography using a hypersil ODS column (250 mm x 4.6 mm, 5pm particle size) and detection at 291 nm. The mobile phase consisted of acetonitrile: phosphate buffer (75:25) which was delivered at 1.5 mL/ min flow rate. The retention time of ETA was found to be 3.8 min. The assay was linear in the concentration range of 0.1-4 µg/ mL. Within day and between day precision extract by relative standard deviation was less than 4 % and inaccuracy did not exceed 3 %. This method is an improved method wherein instead of solid phase extraction, liquid liquid extraction is carried out which resulted in increased sensitivity.

Page(s): 449-452

DOI: DOI not available

Published: Journal: Journal of Chemical Society of Pakistan, Volume: 30, Issue: 3, Year: 2008

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