Abstract:
Serum albumin is crucial in Drug-Pharmacokinetics and pharmacodynamic. So, coordination compounds with therapeutic index needs to be attested by exploring their binding affinity with coordination compounds to qualify for understanding their mechanism of action. Herein drug protein interaction modes will be presented taking into consideration the synthesis, anticancer activity and interaction of trimethyl and triphenyltin(IV) derivatives (labeled as 1 and 2 respectively) of sodium deoxycholate (sodium (4R)-4-((3R,5R,10S,12S,13R,17R)-3,12-dihydroxy-10,13dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-17-yl)pentanoate) with bovine ser and human serum albumin at two different temperatures (298 and 310K) by means of fluorescence quenching experiments. Compounds were well characterized by IR, 1H-, 13C- and 119Sn-NMR. Both the compounds showed good anticancer activity. Fluorescence quenching constants was determined from Stern-Volmer equation. Both the compounds bind with BSA and HSA through dynamic quenching pathway. Compound 1 bind with BSA and HSA at two temperatures with an association constant of 3.9 x 104 and 8.0 x 104 at 298K and 9.9 x 104 and 1.02 x 104 at 310K respectively. Similarly compound 2 binds with BSA and HSA with quenching constants of 3.9 x 104 and 1.8 x 104 at 298K and 1.4 x 104 and 4.5 x 104 respectively. Synchronous fluorescence spectrum of compounds suggested that the tryptophan residues contribute greatly to the quenching of intrinsic fluorescence.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
Anticancer
,
Anticancer
,
Anticancer
,
Anticancer
,
Fluorescence
,
Serum Albumin
,
coordination compounds