The Attenuative Eefcts of Zinc Oxide Nanoparticles and S-Methylmethionine Sulfonium Chloride Against D-Galactose Induced Liver Oxidative Stress and Apoptosis in Albino Rats | [Pakistan Journal of Zoology • 2025]

Author(s):

1. Tarek Kamal Abouzed: Department, Faculty of Veterinary Medicine Kafrelsheikh University,Kafrelsheikh, Egypt

2. Marwa F. Ezzeldeen: Department, Faculty of Veterinary Medicine Kafrelsheikh University,Kafrelsheikh, Egypt

3. Azza Mansour Elkattawy: Department, Faculty of Veterinary Medicine Kafrelsheikh University,Kafrelsheikh, Egypt

4. Ola A. Elbohy: Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.

5. Doaa Abdullah Dorghamm: Department, Faculty of Veterinary Medicine Kafrelsheikh University,Kafrelsheikh, Egypt

Abstract:

D-galactose has been documented to induce a sign of aging and is linked to liver injury. Our study aimed to evaluate the protective effect of zinc oxide nanoparticles and S-Methylmethionine sulfonium chloride against D-galactose-induced liver injury in rats and explores their antioxidant, anti-inflammatory, and anti-apoptotic role. This study was carried out on 40 male Wister albino rats and divided into four groups. In the first group; the normal group did not receive any treatment, 2nd group; rats were injected intraperitoneally with a dose of 50 mg/kg daily of D- galactose for two months, 3rd group; rats received D-galactose at the same dose and received Zinc oxide nanoparticle orally by a dose (10 mg/kg/daily), 4th group; received D-galactose by the same dose and was received S-Methylmethionine by a dose (50mg/kg/daily). The present study revealed that intraperitoneal injection of D-galactose (50 mg/kg/day) leads to a marked loss of body weight, a markedly increase in lipid profile and blood glucose level, a markedly increase in malondialdehyde (MDA) level in the liver, and a markedly decrease in glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) in the liver. D-galactose has a role in elevating hemoglobin A1c (HbA1c), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), albumin, and fructosamine, while in the gene expression, there was an increase in inducible nitric oxide synthase (iNOS), tumor necrosis factor a (TNF-a), transforming growth factor ß (TGF-ß), interleukin 6 (IL-6), BAX gene expression and decrease in Bcl2 gene expression in the liver. Zinc oxide nanoparticles and S-Methylmethionine sulfonium chloride are novel treatments that play an important role in maintaining weight, managing normal lipid profile, blood glucose levels, oxidant, and antioxidant enzymes, HbA1c, ALT, AST, GGT, albumin, and fructosamine. The effect of these substances was extended to the level of gene expression of the tested genes. In conclusion, zinc oxide nanoparticles and S-Methylmethionine attenuated oxidative stress and hepatic damage induced by D-galactose treatment.

Page(s): 1677-1686

DOI: 10.17582/journal.pjz/20230523160530

Published: Journal: Pakistan Journal of Zoology, Volume: 57, Issue: 4, Year: 2025

Keywords:

oxidative stress , antioxidant , Zinc oxide nanoparticles , antioxidant , Antioxidant , Antiaging , Dgalactose , MMSC

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