Plant Microbiome System Development for Bioremediation of Crude Oil Contaminated Soil | [Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 • 2023]

Author(s):

1. Maimona Saeed: Department of Botany, PMAS-Arid Agriculture University Rawalpindi, 46300 Rawalpindi, Pakistan; Department of Botany, GC Women University, Sialkot, Pakistan

2. Noshin Ilyas: Department of Botany, PMAS-Arid Agriculture University Rawalpindi, 46300 Rawalpindi, Pakistan

3. Muhammad Arshad: Department of Botany, PMAS-Arid Agriculture University Rawalpindi, 46300 Rawalpindi, Pakistan

4. Muhammad Sheeraz: Department of Biochemistry, PMAS-Arid Agriculture University Rawalpindi, 46300 Rawalpindi, Pakistan

5. Iftikhar Ahmed: Bio-resources Conservation Institute, National Agriculture Research Centre, Islamabad, Pakistan

6. Arghya Bhattacharya: GIET University Gunupur, Odisha, India

Abstract:

Bacterial assisted phytoremediation is recently being considered to be an efficient technique for remediation of crude oil-contaminated soil. The present research was designed to establish a plant microbiome bioremediation system for treating crude oil contamination. 10 strains of plant growth-promoting rhizobacteria (PGPR) were isolated from oil-contaminated soil near Oil Refinery Rawalpindi, Pakistan. Based on plant growth-promoting characteristics and bio surfactant production, two strains (Pseudoarthrobacter phenanthrenivorans (MS2) and Azospirillumoryzae (MS6)) were selected. They showed a better emulsification index (54.2, 42.5%), oil displacement activity (3.4, 2.6 mm) and hydrophobicity content (78 & 75%). For the establishment of the plant micro biome system, both strains and their combination were inoculated in rhizo spheric soil of maize in crude oil-contaminated soil. Better germination attributes of maize were observed by a combination of both strains with improved fresh (32 %) and dry biomass (26.5 %) as compared to control under oil stress (10 %). Plant micro biome bioremediation system improved the chlorophyll content (30.4 %), water potential (23.2 %), proline (32 %), amino acids (11.1 %), and antioxidant enzymes (catalase (21 %), peroxidase dismutase (30 %) and superoxide dismutase (22 %), as compared to control under oil stress (10 %). The hydrocarbons degradation efficiency of this system was 38.5%. Analysis of degradation products by GC-MS revealed the presence of low molecular weight hydrocarbons in the treated soil as compared to untreated soil. This study showed promising results by this plant micro biome system can be a way forward in bacterial assisted phytoremediation approaches at the field level in the future.

Page(s): 40-40

DOI: DOI not available

Published: Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords:

Antioxidant enzymes , Hydrocarbons , Antioxidant enzymes , consortium , Bio surfactant , oil degradation

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