The Inhibitory effect of Placenta Fetal Mesenchymal stem cells on Prostate cancer cell line | [Bioscience Research • 2024]

Author(s):

1. Aisha Alyamani: Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Cell Culture Unit and Experimental Biochemistry Unit, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia

2. Etimad Huwait: Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Cell Culture Unit and Experimental Biochemistry Unit, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah, Saudi Arabia

3. Samar Sultan: Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Regenerative medicine unit, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah 21589, Saudi Arabia

4. Nouf Alsubhi: Regenerative medicine unit, King Fahad Medical Research Centre, King Abdulaziz University, Jeddah 21589, Saudi Arabia Department of Biological Science, Faculty of Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Embryonic Stem Cell Research Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia; Embryonic and Cancer Stem Cell Research Group, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia

5. Afnan Bahmaid: Department of Biological science, College of Science, University of Jeddah, Jeddah 80327, Saudi Arabia

6. Saleh Alkarim: Biological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia

7. Ghadeer Alrefaei: Department of Biological science, College of Science, University of Jeddah,Jeddah 80327,Saudi Arabia

Abstract:

Mesenchymal stem cells (MSCs) have shown antitumor effects against various cancers. However, the mechanism of PFMSCs and their extracts related to prostate cancer cell lines 22Rv1 remains undetermined. Therefore, the above study investigated the suppressive effects of PF-MSCs extract, including PF-MSCs/CM and PF-MSCs/CL, against 22Rv1 cells in vitro. Firstly, the characteristics of MSCs were investigated, followed by the preparation of PF-MSCs/CM and PFMSCs/CL and the inhibitory effects against 22Rv1 cells. Finally, we used flow cytometry to assess how extracts affect the cell cycle of 22Rv1 cells and real-time polymerase chain reaction to measure the expression of inflammatory genes. Our study uncovered some interesting findings about PF-MSCs: they have the same markers as MSCs, can differentiate into bone and fat cells, and can multiply faster. When we treated the cells with 100% PF-MSCs/CM and 100 µg/ml PFMSCs/CL, we observed an improvement in cell viability. We also noticed that the extracts caused a decrease in the subG1 population. On top of that, specific genes related to cell death, like BAX and CASP3, were more active, while genes associated with inflammation, such as IFN-?, TNF-a, IL-1ß, IL-6, IL-8, and IL-12A, were less active. In contrast, the expression of anti-inflammatory genes like IL-4 and IL-10 increased. Based on our findings, it seems that PF-MSCs can enhance the stimulation of 22Rv1 cells, which might be why they can promote cancer.

Page(s): 680-691

DOI: DOI not available

Published: Journal: Bioscience Research, Volume: 21, Issue: 4, Year: 2024

Keywords:

Prostate cancer , differentiation , gene expression , Mesenchymal Stem Cell , Secretome

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