Therapeutic role of Rauwolfia serpentina in minimizing the risk of glycosylation and associated biomarkers in experimentally induced type 1 diabetic mice | [Pakistan Journal of Pharmaceutical Sciences • 2021]

Author(s):

1. Muhammad Bilal Azmi: Department of Biochemistry, Dow Medical College, Dow University of Health Sciences,Karachi,Pakistan

2. Shamim Akhtar Qureshi: Department of Biochemistry, University of Karachi,Karachi,Pakistan

3. Syed Danish Haseen Ahmed: Department of Biochemistry, Dow Medical College, Dow University of Health Sciences,Karachi,Pakistan

4. Auwais Ahmed Khan: Department of Pharmacology, Dow College of Pharmacy, Dow University of Health Sciences,Karachi,Pakistan

5. Muhammad Ahsan: Department of Biochemistry, Dow Medical College, Dow University of Health Sciences,Karachi,Pakistan

6. Hina Akram Mudassir: Department of Biochemistry, Federal Urdu University of Arts, Science and Technology,Karachi,Pakistan

7. Fauzia Imtiaz: Department of Biochemistry, Dow Medical College, Dow University of Health Sciences,Karachi,Pakistan

8. Sumera Rais: Department of Biochemistry, Federal Urdu University of Arts, Science and Technology,Karachi,Pakistan

Abstract:

Present work investigates the effects of hydro-methanolic roots extract (HyMREt) of Rauwolfia serpentina in type 1 diabetic mice. Mice were divided into normal, diabetic, negative and positive controls (I-IV) and three test (HyMREt doses) groups (V-VII - 50, 100, &150mg/kg). Allocated treatment of each group was given orally for 14 days in overnight fasted state. Percent change in fasting blood glucose (FBG), body weights, body tissue weights, hepatic glycogen, total lipids, glycosylated hemoglobin (HbA1c), complete blood profile and antioxidant enzymes including catalase (CAT) and superoxide dismutase (SOD) were estimated. HyMREt doses produced meaningful (p<0.0001) reduction (-39 to -53%) in FBG. Hemoglobin (Hb) levels were raised, HbA1c were considerably decreased (4.5-3.77%) and glycosylation (HbA1c to Hb) ratio was expressively (p<0.0001) improved in test groups. Dose-wise improvement (p< 0.05) in total glycogen and decrement (p<0.05) in lipids were observed in livers of test groups. HyMREt significantly decreased (p<0.05) percent inhibition of SOD and CAT. HyMREt doses progressively (p<0.05) improved RBC and other hematological parameters while decrement was only noticed in leucocyte counts. Administration of test doses of HyMREt were significantly reduced the glycosylation, oxidative stress and anemia caused by alloxan intoxication in mice.

Page(s): 69-76

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 34, Issue: 1, Year: 2021

Keywords:

Alloxan , Anaemia , catalase , glycosylation , superoxide dismutase , Rauwolfia serpentina

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