Toxicity of proteins secreted by entomopathogenic fungi against Plutella xylostella (Lepidoptera: Plutellidae). | [International Journal of Agriculture and Biology • 2012]

Author(s):

1. Shoaib Freed: University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

2. Feng Liang Jin: University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

3. Shun Xiang Ren: University College of Agriculture, Bahauddin Zakariya University, Multan, Pakistan

4. Muhammad Naeem: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, P.R. China

5. Mubshar Hussian: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, P.R. China

Abstract:

The diamond back moth (DBM) (Plutella xylostella L.) is an important and cosmopolitan pest of cruciferous crops. The toxic crude proteins produced by Isaria fumosorosea were checked against DBM for its effectiveness as insecticidal and antifeedant characteristics. The crude proteins produced in the Czapek Dox liquid medium were used against 3rd instar larvae of DBM. The results indicated a considerable insecticidal and antifeedant activity by crude protein extracts. Notably the most active crude protein extract belong to the isolate CNZH that showed 83.3% mortality of 3rd instar larvae 6 days post treatment. CNZH was tested for its toxicity at different concentrations of crude protein and a maximum mortality percentage of 91.6 were recorded after 6 days of treatment. A significant level of increase in the antifeedant index was recorded with the increase of concentrations and time duration. At the concentration of 9 mg protein/mL the antifeedant index was observed to be significantly higher than that of 6 and 3 mg protein/mL (P<0.05). CNZH due its insecticidal characteristics can be recommended for the incorporation in the biological control program of DBM. Further studies regarding purification of the active microbial insecticidal compound and its mode of action need to be carried out. The complete purification of the protein and its insecticidal and antifeedant tests in the field can help to develop a natural product for plant protection.

Page(s): 291-295

DOI: DOI not available

Published: Journal: International Journal of Agriculture and Biology, Volume: 14, Issue: 2, Year: 2012

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