Use of an optimal and distinctive method for the extraction of genomic DNA from Juniperus excelsa of the Zarghoon juniper ecosystem in Balochistan, Pakistan. | [Pure and Applied Biology • 2015]

Author(s):

1. Zahoor Ahmed Bazai: Department of Botany, University of Balochistan, Quetta (87300), Pakistan

2. Rasool Bakhsh Tareen: Department of Botany, University of Balochistan, Quetta (87300), Pakistan

3. Muhammad Younas Khan Barozai: Department of Botany, University of Balochistan, Quetta (87300), Pakistan

4. Humma Batool: Department of Botany at the SBK, Women University, Quetta (87300) Pakistan

5. Abdul Kabir Khan Achakzai: Department of Botany, University of Balochistan, Quetta (87300), Pakistan

6. Basira Akhtar: Department of Botany, University of Balochistan, Quetta (87300), Pakistan

Abstract:

This exploration was carried out through field inspection in immediate forest neighborhoods of two settlements entitled Killi Tor Shore (K.T.S.) and Killi Shaban (K.S.) in revision region Zarghoon Juniper ecosystem, three districts named Quetta, Sibi and Ziarat, province Balochistan, Pakistan. The leaf samples of Juniperus excelsa trees were collected from these areas and subjected for the DNA isolation. As no genetic analysis is reported for this area Juniper, thus this study is aimed to optimize a protocol for the extraction of DNA. In order to isolate high aggregate of pure and intact DNA would serve great base for the downstream genetic analyses. The impurities of plants predominantly the pine trees are owing to the existence of high magnitude of secondary metabolites, polyphenols and polysaccharides. After the application of different acknowledged protocols for the DNA extraction of Juniperus excelsa trees it was perceived none of the protocols extracted pure and high yield DNA. Therefore, with the adaptation in technique an optimized protocol abetted in isolating pure DNA chiefly for tree Juniperus excelsa. This optimized protocol applied multiple times consequently made possible the isolation of pure DNA of Juniper.

Page(s): 659-663

DOI: DOI not available

Published: Journal: Pure and Applied Biology, Volume: 4, Issue: 4, Year: 2015

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