Abstract:
Diabetes mellitus (DM) is a group of metabolic disorders that can lead to various complications such as blindness, amputation, and renal failure. Unhealthy and sedentary lifestyles contribute to obesity and the increasing prevalence of diabetes worldwide. DM can occur due to the absence or insensitivity of insulin, leading to hyperglycemia. Type 1 diabetes mellitus (T1DM) results from the destruction of pancreatic beta cells responsible for insulin production, while type 2 diabetes mellitus (T2DM) is characterized by insulin resistance in body cells. Gestational diabetes occurs during pregnancy and causes glucose intolerance in the third trimester. DM is associated with both microvascular and macrovascular complications. Peripheral arterial disease (PAD) is one of the macrovascular complications characterized by obstruction of blood flow, especially to the lower extremities. The pathogenesis of DM involves inflammation, which leads to insulin resistance and impaired glucose tolerance. Inflammatory biomarkers such as ICAM-1 are associated with DM and its complications, including PAD. This research involved collecting serum from subjects divided into three groups: 8 subjects with DM, 8 with DM+PAD, and 2 healthy subjects in the control group. The enzyme-linked immunosorbent assay (ELISA) technique was used to detect soluble ICAM-1 (sICAM-1) levels in the different groups, and absorbance measurements were taken using the Biotek ELx800. The results were presented in the form of histograms, confirming the highest sICAM-1 levels in group 2 (DM+PAD), moderate levels in group 1 (DM), and the lowest levels in the control group. Genderbased analysis was performed within each group, considering variables such as age. It was found that inflammation was higher in older individuals, with no apparent link to gender. These findings suggest that sICAM-1 levels are elevated in individuals with DM and PAD, indicating the presence of inflammation and its potential contribution to the development and progression of PAD in diabetic patients. Further research is necessary to explore the underlying mechanisms and potential therapeutic strategies targeting inflammation and ICAM-1 in the management of DM and its complications.
Page(s):
386-386
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023