Simultaneous HPLC quantification of Diltiazem and n-demethyldiltiazem in plasma. | [Pakistan Journal of Pharmacology • 2006]

Author(s):

1. Nisar-Ur-Rahman: Department of Pharmacy, Islamia University Bahawalpur, Pakistan

2. S. S. Hasan: Department of Pharmacy, Islamia University Bahawalpur, Pakistan

3. Uzma Niaz: University College of Pharmacy, University of the Punjab, Lahore, Pakistan

Abstract:

A simple and sensitive reversed phase high-performance liquid chromatographic method was developed for simultaneous quantification of diltiazem and its major metabolite N-demethyldiltiazem in human plasma. The method involves one-step solvent extraction of diltiazem, N-demethyldiltiazem and the internal standard, verapamil with n-hexane and diethyl ether (50:50 v/v). The mobile phase comprised 0.1M ammonium dihydrogen phosphate-acetonitrile (62:38 v/v) and triethylamine (0.08%) was added before the pH was adjusted to 5.9 with 85% phosphoric acid. Analysis was run at a flow rate of 1.0 ml/mm at a detection wavelength of 238 nm. The completion time for assay was not more than 10 minutes and lower limit of quantification was 5 ng/ml. The calibration curve for diltiazem and its metabolite was linear over a concentration range of 5-200 ng/ml and average recovery was about 90%. The coefficient of variation and percent error values of the assay method within and between day were all less than 10%.

Page(s): 61-65

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmacology, Volume: 23, Issue: 1, Year: 2006

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