Whole Genome Sequencing Insights on Extensive Drug Resistant Klebsiella pneumoniae and Pseudomonas aeruginosa traumatic infection | [Pakistan Journal of Biological Sciences • 2025]

Author(s):

1. Hekmat Ahmed Owaid: Department of Biology, College of Science, University of Anbar, Al-Anbar Governorate, Ramadi, Iraq

2. Mushtak Talib Salih Al-Ouqaili: Department of Microbiology, College of Medicine, University of Anbar, Al-Anbar Governorate, Ramadi, Iraq

Abstract:

Background and Objective: It is well documented that Whole Genome Sequencing (WGS) has recently used to explore new resistance patterns and track the dissemination of extensive and pan drug-resistant microbes in healthcare settings. This article explores the link between traumatic infections caused by road traffic accidents (RTAs) leadingt o coma and the development of chest infections caused by extensively drug-resistant (XDR) Klebsiella pneumoniae and Pseudomonas aeruginosa. Matearnd iMeatholds:s T he study was carried out from March to December 2022 which included a 45-year-old male patient admitted to the ICU of Al Ramadi Teaching Hospitals followinga severe RTA that resulted ina TBl and subsequent coma. Twostudyisolatweesre diagnosed bacterioluosigng itche aVlITlEKy®- 2 technique including resistant mechanisms like extended-spectrum beta-lactamases and carbapenemases. Whole genome sequencing was performed using a DNA nanoball sequencing platform from BGI-Tech. Genome assembly and annotation were done using the bacterial bioinformatics resource center. The report on Comprehensive Genome Analysis includes a phylogenetic analysis using the reference and representative genomes provided by PATRIC. Results: Klebsiella pneumoniae and P. aeruginosa isolates were XDR, producing ESBLs and carbapenemases. The WGS detection NDM-5 gene in the K. pneumoniae strain is not very common compared to the NDM-1 and blaOXA-181 g. At the same time, a file in 2. aeruginosa isolate found genes GES-type ESBL (not reported in Iraq before), blaPAO and blaOXA-396 with NDM-1 all these genes are carbapenemases. In phylogenetic analysis, the A pneumoniae isolate has an evolutionary relationshwiitph strains originating from China while 2. aeruginosa was globally unique. Conclusion: The XDR K pneumoniae and P. aeruginosa pose a public health threat. The WGS revealed unique virulence and antibiotic-resistance genes associated with nosocomial outbreaks. The XDR isolates carrying NDM-5, blaOXA-181 and GES-type ESBL genes were detected.

Page(s): 78-87

DOI: 10.3923/pjbs.2025.78.87

Published: Journal: Pakistan Journal of Biological Sciences, Volume: 28, Issue: 2, Year: 2025

Keywords:

Klebsiella pneumoniae , Road Traffic Accidents , Pseudomonas aeruginosa , Ventilatorassociated pneumonia , whole genome sequencing

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