The Influence and Mechanism of Benzodihydropyran Benzene and Dihydro Pyran Derivatives xy2004 effect on Breast Cancer Cell Proliferation and its Mechanism Ramification. | [RADS Journal of Pharmacy & Pharmaceutical Sciences • 2016]

Author(s):

1. Li Cui: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou city, PR China

2. Miaomiao Sun: Department of Pathology, Henan Cancer Hospital, Zhengzhou city, PR China

3. Zhihua Zhao: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou city, PR China

4. Jianping Yang: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou city, PR China

5. Yupeng Zheng: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou city, PR China

6. Lulu He: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou city, PR China

7. Kuisheng Chen: Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou city, PR China

8. Qingxia Fan: Medical oncology, eTh First Afiliated Hospital of Zhengzhou University, Zhengzhou city, PR China

Abstract:

Objective To investigate the eefcts of xy2004 on the proliferation of MCF-7 cells and its mechanism. Methods Eefcts of xy2004 on the proliferation of MCF-7 cells was determined by MTT assay. Cell apoptosis was examined by flow cytometry. Apoptosis proteins were examined by western blot Competitive estrogen-receptor binding assay was used to investigate the afinity of xy2004 ER. Results xy2004 induced cell proliferation of MCF-7cells in low concentrations and inhibited cell proliferation in high concentrations. Antiestrogen tamoxifen eliminated the stimulation of proliferation of MCF-7 induced by xy2004. The relative binding afinity of xy9906 for ERa and ERß showed an IC50 of 7.38×10-5M and 4.12×10-7M respectively. Aeftr MCF-7 cells were treated with xy2004 in high concentration, Bcl-2 protein expressions were reduced and Bax protein expressions were increased. Conclusion xy2004 directly stimulates cell proliferation of MCF-7 cells through ligand-receptor binding in low concentrations and inhibited cell proliferation in high concentrations through regulating the apoptosis proteins.

Page(s): 21-27

DOI: DOI not available

Published: Journal: RADS Journal of Pharmacy & Pharmaceutical Sciences, Volume: 4, Issue: 1, Year: 2016

Keywords:

MCF7 , benzodihydropyran , ER , proliferation

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