To estimate effective antiamyloidogenic property of melatonin and fisetin and their actions to destabilize amyloid fibrils | [Pakistan Journal of Pharmaceutical Sciences • 2017]

Author(s):

1. Mohammad Hossein Aarabi: Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences,Kashan,Iran

2. Seyyed Mehdi Mirhashemi: Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran; Clinical Biochemistry and Genetics Department, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran

Abstract:

Aggregating of amylin as pancreatic deposition is connected with pancreas degeneration in type 2 diabetes mellitus. Suppression of the amylin accumulation and so instability of the pre-formed pancreatic ß-amyloid, may be attractive curative goal for mediation of diabetes mellitus. Fluorimetric assay by Thioflavin-T was utilized for investigating the properties of melatonin and fisetin on the generation and instability of ß-amyloid near to physiological conditions. The results showed that after 168 hours incubation by shaker incubator in 37oC, melatonin at 10µM and 40 µM repressed amylin amyloid formation by 20.1% and 27.5% respectively (p<0.05) and the similar values of fisetin inhibited the formation of ß-sheet structure by 16.5% and 23.2% respectively (p<0.05).The obtained data also confirmed that amyloidal sheet opening was induced by melatonin and fisetin significantly (p<0.05). It may be concluded that islet amyloid cytotoxicity to ß-cells may be reduced by melatonin and fisetin, and they should be important constituents of new drugs for diabetes mellitus treatment.

Page(s): 1589-1593

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 30, Issue: 5, Year: 2017

Keywords:

Diabetes Mellitus , melatonin , Amylin fibrils , Fisetin , Amylin

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