Author(s):
1. Anmol:
Department of Biological Sciences, UVAS-Lahore, Ravi Campus, Pattoki, Pakistan
2. Saira Ishaq:
Department of Chemistry, Government Islamia Graduate College, Lahore, Pakistan
3. Aamna Ishaq:
Department of Biological Sciences, UVAS-Lahore, Ravi Campus, Pattoki, Pakistan
Abstract:
Aspergillus fumigatus Fresen., is the most prevalent species in the genus. A. fumigatus is the leading cause of invasive aspergillosis and it is the most common cause of superficial infection. It also contributes to significant morbidity and mortality in immunosuppressed patients. Grephane Oxide Nickel Ferrite (GrONiF) nanoparticles were chemical method. The prepared GrONiF nanoparticles were evaluated for their antifungal activity against A. fumigatus. The antifungal activity was observed as inhibition in spore germination and by determining the zone of inhibition of fungal pathogen. Effect of concentration of GrONiF nanoparticles on culture media plates was observed. The antifungal activity was observed by modified agar disk diffusion and well methods; broth recommended by Clinical Laboratory Standards Institute (CLSI, 2011). Approximately 10^8 colony forming units (CFU) of A. fumigatus were inoculated in 10mL PBS buffer solution, hence suspension solution prepared. Culture media plates were prepared by sterilized Potato Dextrose Agar (PDA) media by pouring 20ml media into each empty sterilized petri plates. Activity index was recorded after 12, 24, 36 and 48h incubation at 37°C, The diameter of the inhibition of zones were measured and optical images were documented by an ordinary camera at the same time. Results convinced that GrONiF nanoparticles enhanced and lengthened antifungal activity. GrONiF are concluded to have the great potential in applications such as clinical nosocomial infections and antifungal therapy.
Page(s):
173-173
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
antimicrobial
,
antimicrobial
,
Nanoparticals
,
Biofungicides
References:
References are not available for this document.
Citations
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