Variations in Nucleotide Frequency Distribution in Spider Families | [Punjab University Journal of Zoology • 2019]

Author(s):

1. Sehrish Ashraaf: Department of Zoology, Government College University, Lahore, Pakistan

2. Hafiz Muhammad Tahir: Department of Zoology, Government College University, Lahore, Pakistan.

3. Muhammad Akram Qazi: Punjab Agriculture Research Board, Imam Hussain Road, Gulberg III, Lahore, Pakistan

4. Shaukat Ali: Department of Zoology, Government College University, Lahore, Pakistan.

Abstract:

| The study was designed to test the hypothesis, variations in nucleotide frequency distribution in cytochrome c oxidase subunit I (COI) in spiders used as a marker to delimit species. Based on morphological identification and sequencing of CO1 gene, a total of 22 species of spiders belonging to five families were investigated and compared within each species and among families for nucleotide frequency distribution of all species collected. We had found non-significant diference in frequency of nucleotides in COI within the members of species and within families (thymine 41.30-44.30%, adenine 24.96-27.19%, guanine 17.18-19.48% and cytosine 12.28-13.40% while GC 29.53-32.84%). Therefore, it was concluded that variation in nucleotide frequency distribution could not to bee used as a criterion for species delimitation in spiders.

Page(s): 101-104

DOI: 10.17582/journal.pujz/2019.34.1.101.104

Published: Journal: Punjab University Journal of Zoology, Volume: 34, Issue: 1, Year: 2019

Keywords:

Biomarker , Spiders , Frequency distribution , Nucleotides

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