Author(s):
1. Noman Razzaq:
The Islamia University of Bahawalpur, Bahawalpur, Pakistan
2. Syed Ali Raza Naqvi:
The Islamia University of Bahawalpur, Bahawalpur, Pakistan
3. Saima Taiq:
The Islamia University of Bahawalpur, Bahawalpur, Pakistan
Abstract:
Radiopharmaceuticals contain radionuclide that are used for diagnostic and therapeutic purposes. Cephradine is first generation cephalosporin used to treat infection caused by both Gram-positive and Gram-negative bacteria such as urinary tract infection, upper respiratory infection, skin infection and sinusitis. Accumulation of cephradine at the site of lesion is very low and bacterial resistance also lower down its effectiveness. The purpose of this work is to label the cephradine with 99mTc to treat and diagnose deep-seated infections. The optimized parameters such as amount of reducing agent, concentration of cephradine, reaction time and pH was studied on the labeling yield of 99mTc-cephradine complex. The labeling yield of the desired complex was almost 90 % by using 1.5 mg of cephradine; 150 µg SnCl2.2H2O at pH 5 and 30 min reaction time. Radiolabeled complex was characterized by using instant thin layer chromatography. The formed complex was stable up to the period of 24 h during which labeling yield decrease only 5 %. Biodistribution was also study in both normal and infected mice with Staphylococcus aureus bacteria. Both thighs of the infected mice were dissected and T/NT ratio for 99mTc-cephradine complex were 1.86 ± 0.51 and 3.11 ± 1.00 after one and three hours of post injection.
Page(s):
49-49
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023
Keywords:
Radio synthesis
,
biological evaluation
,
drug loaded nanoparticles
References:
References are not available for this document.
Citations
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