A novel hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase (HPPK-DHPS) gene from a nutraceutical plant seabuckthorn, involved in folate pathway is predominantly expressed in fruit tissue. | [International Journal of Agriculture and Biology • 2016]

Author(s):

1. Shazia Arif: Faculty of Agriculture Rawalakot Poonch, Azad Jammu and Kashmir University, Muzaffarabad, Azad Kashmir, Pakistan

2. M. Ramzan Khan: National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Islamabad, Pakistan

3. Syed Dilnawaz Ahmed Gardezi: Faculty of Agriculture Rawalakot Poonch, Azad Jammu and Kashmir University, Muzaffarabad, Azad Kashmir, Pakistan

4. Zaheer Abbas: National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Islamabad, Pakistan

5. Ghulam Muhammad Ali: National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre (NARC), Islamabad, Pakistan

Abstract:

Seabuckthorn (Hippophae rhamnoides L.) is a hardy fruit-producing plant. With medicinal and nutraceutical properties, its fruits, seeds and leaves are enriched with astonishing array of nutrients. Especially, its bark contains vitamins, essential oils and minerals, for which most of the staple crops are deficient. This study was envisaged to isolate the folate pathway HPPKDHPS ortholog from seabuckthorn. The target gene Hydroxymethyldihydropterin pyrophosphokinase–dihydropteroate synthase linked in the pathway of folate biosynthesis was successfully isolated and cloned. The sequences analysis revealed that this novel genomic locus is 2354 bp in size. The coding region is interrupted by a single large intron. The coding sequence is 1539 bp long which is similar to its ortholog in tomato. The newly isolated gene has prominent nucleotide mutations randomly distributed over the entire length of the sequence. Mutations are only of substitution type, and no deletions or insertions are detectable. All the mutations were of non-synonymous type. Expression profile of HrHPPK-DHPS with semiquantitative RT-PCR revealed the higher accumulation of transcripts in leaf and fruits tissues. Phylogenetic reconstruction revealed gene duplication in soybean and Arabidopsis orthologs. A considerable conservation in 3-dimensional structure of folate protein HPPK-DHPS was observed; however, notable differences in substrate binding pockets were also visible. Hence, HrHPPK-DHPS is a novel ortholog isolated from medicinal plant seabuckthorn.

Page(s): 412-418

DOI: DOI not available

Published: Journal: International Journal of Agriculture and Biology, Volume: 18, Issue: 2, Year: 2016

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