Trials for Preparation and Evaluation of Combined Inactivated Mycoplasma Gallisepticum and SynoviaeVaccine in Chicken and Turkey | [International Journal of Veterinary Science • 2023]

Author(s):

1. Marwa M.S. Khedr: Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC),Abbasia, Cairo,Egypt

2. Gina M. Mohamed: Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC),Abbasia, Cairo,Egypt

3. Marwa Fathy El Sayed: Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC),Abbasia, Cairo,Egypt

4. Heba M. Soliman: Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC),Abbasia, Cairo,Egypt

5. Nayera M. Alatfeehy: Department of Bacteriology, Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute (AHRI), Agricultural Research Center (ARC),Dokki, Giza,Egypt

6. Mounir Mohamed Diab El Safty: Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC),Abbasia, Cairo,Egypt

Abstract:

A large number of poultry farms around the world have been shut down during the last few years due to avian Mycoplasmosis, especially those where chickens and turkeys were raised. Therefore, the main goal of this study was to investigate the possibility of eradicating the disease by immunizing one-week-old chicks and turkey poults against Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) using an inactivated single dose or a combined vaccine. In this study, a total of 280 one-week-old Specific-Pathogen-Free chicks were divided into four groups to evaluate their immune response following vaccination. Chicks of group 1 (n=60) were given inactivated MG vaccine, those of group 2 (n=60) were given inactivated MS vaccine. Chicks of group 3 (n=120) received combined inactivated MG+MS vaccine, while birds of group 4 (n=40) served as control. After three weeks, the chicks were administered with the booster dose of the respective vaccine. Similarly, 60 turkey poults of one week in age were divided into two groups: Poults of group 1 (n=50) received combined inactivated MG+MS vaccine, while those of group 2 (n=10) were kept as control. The booster dose of vaccine in group 1 birds was given after 4 weeks. The immune responses of vaccinated chicks and turkey poults were measured by HI test, ELISA and challenge test. The results of this study revealed that the combined inactivated MG+MS vaccine adjuvanted with Montanite ISA70 was more effective against Mycoplasma synoviae and Mycoplasma gallisepticum in chickens and turkey poults than the individual inactivated vaccine and there was significant difference in the group of turkey poult vaccinated with combined inactivated MGMS in ELISA test.

Page(s): 545-553

DOI: 10.47278/journal.ijvs/2023.004

Published: Journal: International Journal of Veterinary Science, Volume: 12, Issue: 4, Year: 2023

Keywords:

ELISA , HI Test , Mycoplasmosis , Inactivated Vaccines

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